EXPRESSION OF THE YEAST LEU4 GENE IS SUBJECT TO 4 DIFFERENT MODES OF CONTROL

被引：17

作者：

PETERS, MH ^{[1
]}

BELTZER, JP ^{[1
]}

KOHLHAW, GB ^{[1
]}

机构：

[1] PURDUE UNIV,DEPT BIOCHEM,W LAFAYETTE,IN 47907

来源：

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS | 1990年 / 276卷 / 01期

关键词：

D O I：

10.1016/0003-9861(90)90041-V

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A translational fusion of yeast LEU4 and Escherichia colilacZ which contains 679 bp of the LEU4 5′-flanking region and the first two codons of LEU4 was used to study LEU4 expression. Eight recipient strains with different genetic backgrounds, transformed with a plasmid containing the fusion, were grown under a variety of conditions, and β-galactosidase activity was measured. Evidence was obtained for at least four modes of expression of LEU4: general amino acid control, leucine-specifc control, basal level expression, and branched-chain amino acid-mediated repression. Determination of steady-state levels of LEU4 mRNA suggested that LEU4 expression is regulated transcriptionally. © 1990.

引用

页码：294 / 298

页数：5

共 28 条

[1] A SUPPRESSOR OF A HIS4 TRANSCRIPTIONAL DEFECT ENCODES A PROTEIN WITH HOMOLOGY TO THE CATALYTIC SUBUNIT OF PROTEIN PHOSPHATASES [J].