IDENTIFICATION OF A MISLABELED FIXED SPECIMEN BY DNA ANALYSIS

被引：23

作者：

SHIBATA, D ^{[1
]}

NAMIKI, T ^{[1
]}

HIGUCHI, R ^{[1
]}

机构：

[1] CETUS CORP, EMERYVILLE, CA 94608 USA

来源：

AMERICAN JOURNAL OF SURGICAL PATHOLOGY | 1990年 / 14卷 / 11期

关键词：

Fixed tissue; HLA; Mislabeled specimens; Nonradioactive detection; Polymerase chain reaction;

D O I：

10.1097/00000478-199011000-00009

中图分类号：

R36 [病理学];

学科分类号：

100104 ;

摘要：

Formalin-fixed, paraffin embedded surgical specimens can be identified by genotypic analysis. DNA was extracted from single sections of fixed tissue and amplified by the polymerase chain reaction for the human leukocyte antigen (HLA) DQ α and low density lipoprotein receptor genes. These two loci are polymorphic and fixed specimens can be distinguished based on differences between these alleles. The examination can be performed in 2 days and the HLA DQ α test utilized a nonisotopic color detection method. In this case report, a mislabeled specimen was identified as a section from a mastectomy because they had identical genotypes. Molecular genetic analysis of the DNA present in fixed specimens can yield information not otherwise evident from the histology.

引用

页码：1076 / 1078

页数：3

共 15 条

[1]

DUBEAU L, 1986, CANCER RES, V46, P2964

[2]

EISENSTEIN BI, 1990, NEW ENGL J MED, V322, P178

[3] PURIFICATION OF DNA FROM FORMALDEHYDE FIXED AND PARAFFIN EMBEDDED HUMAN-TISSUE [J].