ALW26I, ECO31I AND ESP3I - TYPE-IIS METHYLTRANSFERASES MODIFYING CYTOSINE AND ADENINE IN COMPLEMENTARY STRANDS OF THE TARGET DNA

被引：26

作者：

BITINAITE, J ^{[1
]}

MANELIENE, Z ^{[1
]}

MENKEVICIUS, S ^{[1
]}

KLIMASAUSKAS, S ^{[1
]}

BUTKUS, V ^{[1
]}

JANULAITIS, A ^{[1
]}

机构：

[1] INST BIOTECHNOL FERMENTAS,GRAICIUNO 8,VILNIUS 2028,LITHUANIA,USSR

来源：

NUCLEIC ACIDS RESEARCH | 1992年 / 20卷 / 19期

关键词：

D O I：

10.1093/nar/20.19.4981

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The specificity of three DNA methyltransferases M.Alw26I, M.Eco31I and M.Esp3I, isolated from Acinetobacter Iwoffi RFL26, Escherichia coli RFL31 and Hafnia alvei RFL3+, respectively, was determined. All the enzymes methylate both strands of asymmetric recognition sites yielding m5C in the top-strand and M6A in the bottom-strand, as below: 5'-GTm5CTC 5'-GGTm5CTC 5'-CGTm-5CTC 3'-Cm6AGAG 3'-CCM6AGAG 3'-GCM6AGAG (M.Alw26I) (M.Eco31I) (M.Esp3I) They are the first members of type IIs methyltransferases that modify different types of nucleotides in the recognition sequence.

引用

页码：4981 / 4985

页数：5

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