EFFICIENT ADENOVIRUS-MEDIATED GENE-TRANSFER INTO HUMAN BLOOD MONOCYTE-DERIVED MACROPHAGES

被引：59

作者：

HADDADA, H ^{[1
]}

LOPEZ, M ^{[1
]}

MARTINACHE, C ^{[1
]}

RAGOT, T ^{[1
]}

ABINA, MA ^{[1
]}

PERRICAUDET, M ^{[1
]}

机构：

[1] CNTS, INSERM, U76, F-75012 PARIS, FRANCE

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1993年 / 195卷 / 03期

关键词：

D O I：

10.1006/bbrc.1993.2168

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The efficiency of gene transfer into human blood inonocyte-derived macrophages has been evaluated using a replication-defective adenovirus vector harboring a lac Z gene of E. coli as a reporter gene. Whereas, no β-galactosidase activity was found in freshly infected purified monocytes, 40% to 80% of infected macrophages which derived from these monocytes showed a β-galactosidase activity, 2 to 4 days after infection and lasted for at least 3 weeks. Moreover, β-galactosidase activity was found in infected monocyte/macrophages 7 days after their injection into a human tumor preestablished in nude mice. These data indicate that it is possible to transfer and stably express a gene of potential therapeutic function into human monocyte-derived macrophages using an adenovirus vector. © 1993 Academic Press, Inc.

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页码：1174 / 1183

页数：10

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