MOLECULAR-CLONING AND NUCLEOTIDE-SEQUENCE OF THE GENE ENCODING CHITINASE-II FROM AEROMONAS SP NO. 10S-24

被引：30

作者：

UEDA, M ^{[1
]}

KAWAGUCHI, T ^{[1
]}

ARAI, M ^{[1
]}

机构：

[1] UNIV OSAKA PREFECTURE, COLL AGR, DEPT APPL BIOL CHEM, SAKAI, OSAKA 593, JAPAN

来源：

JOURNAL OF FERMENTATION AND BIOENGINEERING | 1994年 / 78卷 / 03期

关键词：

D O I：

10.1016/0922-338X(94)90291-7

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A gene encoding chitinase II from Aeromonas sp. no. 10S-24 was cloned into Escherichia coli DH5 alpha by using pUC19 and its nucleotides were sequenced. The structural gene consisted of 1626 bp encoding 542 amino acid residues with a characteristic signal peptide. A typical promoter sequence and Shine-Dalgarno (SD) region were located upstream of the initiation ATG codon. The deduced amino acid sequence of the cloned chitinase II. showed sequence homology with chitinase from Saccharopolyspora erythraea (26%). The chitinase II had Pro-Thr- rich domains, consisting of repeat sequences of 47 amino acids, that are not conserved in other chitinases from bacteria. Generally, repeat sequences of amino acids rich in Pro and/or Thr appear to link discrete functional domains in many cellulases and xylanases.

引用

页码：205 / 211

页数：7