THE CONTENT OF FREE AMINO-ACIDS IN THE HUMAN DUODENAL MUCOSA

被引:22
作者
AHLMAN, B
LEIJONMARCK, CE
WERNERMAN, J
机构
[1] Department of Surgery, Karolinska Hospital Stockholm
[2] Department of Surgery, St Göran's Hospital Stockholm
[3] Department of Anaesthesiology and Intensive Care, St Göran's Hospital Stockholm
[4] Metabolic Research Laboratory, St Göran's Hospital, Stockholm
关键词
D O I
10.1016/0261-5614(93)90044-5
中图分类号
R15 [营养卫生、食品卫生]; TS201 [基础科学];
学科分类号
100403 ;
摘要
The free amino acid concentrations and the total protein content of the duodenal mucosa were determined in biopsy specimens obtained during endoscopic examinations in 10 healthy subjects. The amino acids were separated and quantified by ion exchange chromatography using fluorescence detection. The protein content was analysed according to Lowry. The amino acid pattern found in the duodenal mucosa was quite different from that in the plasma. The total amounts of all individual free amino acids were considerably higher in the mucosa than in the plasma (16.2 +/- 0.6 mmol/kg biopsy weight compared to 2.4 +/- 0.1 mmol/l). Taurine, glutamate and aspartate constituted more then 65% of the total content of all amino acids in the mucosa. Glutamine, the most abundant amino acid in plasma (21%), ranked only as sixth in the duodenal mucosa (4%); still, the absolute concentrations were quite similar in the mucosa and plasma (0.60 +/- 0.05 mmol/kg vs. 0.53 +/- 0.02 mmol/l). This study demonstrates the possibility of determining free amino acids in endoscopic biopsy specimens from the human duodenum. The technique is recommended for repeated sampling in clinical studies on the amino acid metabolism of the intestinal mucosa.
引用
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页码:266 / 271
页数:6
相关论文
共 18 条
  • [11] Ahlman, Leijonmarck, Lind, Et al., Free amino acids in biopsy specimens from the human colonic mucosa, J Surg Res, (1993)
  • [12] Ollenschlager, Langer, Roth, Et al., Amino acid determination of human intestinal mucosa obtained by endoscopic biopsies, Amino acids: Chemistry, Biology and Medicine, pp. 920-927, (1990)
  • [13] Lowry, Rosebrough, Farr, Randall, Protein measurement with the folin phenol reagent, J Biol Chem, 193, pp. 265-275, (1951)
  • [14] Kedenburg, A lithium buffer system for single-column amino acid analysis in physiological fluids, Anal Biochem, 40, pp. 35-42, (1971)
  • [15] Colton, Statistics in Medicine, (1974)
  • [16] Hammarqvist, Stromberg, von der Decken, Et al., Biosynthetic Human Growth Hormone preserves both muscle protein synthesis and the decrease in muscle-free glutamine, and improves whole-body nitrogen economy after operation, Ann Surg, 216, pp. 184-191, (1992)
  • [17] Bergstrom, Muscle electrolytes in man, Scandinavian Journal of Clinical and Laboratory Investigation, 14, pp. 11-13, (1962)
  • [18] Nakshabendi, Obeida, Russel, Et al., Measurement of duodenal mucosal protein synthesis after delivery of [<sup>13</sup>C] leucine and [<sup>13</sup>C] valine by IV and IG routes, Clinical Nutrition, 11, (1992)