ADAPTATION OF MITOCHONDRIAL ATP PRODUCTION IN HUMAN SKELETAL-MUSCLE TO ENDURANCE TRAINING AND DETRAINING

被引:153
作者
WIBOM, R
HULTMAN, E
JOHANSSON, M
MATHEREI, K
CONSTANTINTEODOSIU, D
SCHANTZ, PG
机构
[1] UNIV COLL PHYS EDUC,S-11486 STOCKHOLM,SWEDEN
[2] KAROLINSKA INST,DEPT CLIN CHEM 2,S-14186 HUDDINGE,SWEDEN
[3] KAROLINSKA INST,DEPT PHYSIOL 3,S-14186 HUDDINGE,SWEDEN
关键词
BETA-OXIDATION; CARBOHYDRATE METABOLISM; FAT METABOLISM; LUMINESCENCE; MAXIMAL OXYGEN UPTAKE; OXIDATIVE ENZYMES; OXIDATIVE PHOSPHORYLATION;
D O I
10.1152/jappl.1992.73.5.2004
中图分类号
Q4 [生理学];
学科分类号
071003 ;
摘要
The adaptation of mitochondrial ATP production rate (MAPR) to training and detraining was evaluated in nine healthy men. Muscle samples (approximately 60 mg) were obtained before and after 6 wk of endurance training and after 3 wk of detraining. MAPR was measured in isolated mitochondria by a bioluminometric method. In addition, the activities of mitochondrial and glycolytic enzymes were determined in skeletal muscle. In response to training, MAPR increased by 70%, with a substrate combination of pyruvate + palmitoyl-L-carnitine + alpha-ketoglutarate + malate, by 50% with only pyruvate + malate, and by 92% with palmitoyl-L-carnitine + malate. With detraining MAPR decreased by 12-28% from the posttraining rate (although not significantly for all substrates). No differences were found when MAPR was related to the protein content in the mitochondrial fraction. The largest increase in mitochondrial enzyme activities induced by training was observed for cytochrome-c oxidase (78%), whereas succinate cytochrome c reductase showed only an 18% increase. The activity of citrate synthase increased by 40% and of glutamate dehydrogenase by 45%. Corresponding changes in maximal O2 uptake were a 9.6% increase by training and a 6.0% reversion after detraining. In conclusion, both MAPR and mitochondrial enzyme activities are shown to increase with endurance training and to decrease with detraining.
引用
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页码:2004 / 2010
页数:7
相关论文
共 38 条
  • [31] SCHANTZ PG, 1986, ACTA PHYSIOL SCAND, V128, P1
  • [32] SCHMIDT E, 1974, METHOD ENZYMAT AN, P650
  • [33] AN ELECTRON-TRANSPORT SYSTEM ASSOCIATED WITH OUTER MEMBRANE OF LIVER MITOCHONDRIA - A BIOCHEMICAL AND MORPHOLOGICAL STUDY
    SOTTOCASA, GL
    KUYLENSTIERNA, B
    ERNSTER, L
    BERGSTRAND, A
    [J]. JOURNAL OF CELL BIOLOGY, 1967, 32 (02) : 415 - +
  • [34] THE INFLUENCE OF ANOXIA ON LACTATE UTILIZATION IN MAN AFTER PROLONGED MUSCULAR WORK
    STROM, G
    [J]. ACTA PHYSIOLOGICA SCANDINAVICA, 1949, 17 (04): : 440 - 451
  • [35] SUBCELLULAR-DISTRIBUTION AND PROPERTIES OF ALDEHYDE DEHYDROGENASES IN RAT-LIVER
    TOTTMAR, SOC
    PETTERSSON, H
    KIESSLING, KH
    [J]. BIOCHEMICAL JOURNAL, 1973, 135 (04) : 577 - 586
  • [36] ACTIVATION OF HUMAN-MUSCLE PYRUVATE-DEHYDROGENASE WITH ACTIVITY AND IMMOBILIZATION
    WARD, GR
    MACDOUGALL, JD
    SUTTON, JR
    TOEWS, CJ
    JONES, NL
    [J]. CLINICAL SCIENCE, 1986, 70 (02) : 207 - 210
  • [37] A SENSITIVE METHOD FOR MEASURING ATP-FORMATION IN RAT MUSCLE MITOCHONDRIA
    WIBOM, R
    LUNDIN, A
    HULTMAN, E
    [J]. SCANDINAVIAN JOURNAL OF CLINICAL & LABORATORY INVESTIGATION, 1990, 50 (02) : 143 - 152
  • [38] WIBOM R, 1990, AM J PHYSIOL, V259, P504