THE DNA POLYMERASE-ENCODING GENE OF AFRICAN SWINE FEVER VIRUS - SEQUENCE AND TRANSCRIPTIONAL ANALYSIS

被引：31

作者：

RODRIGUEZ, JM ^{[1
]}

YANEZ, RJ ^{[1
]}

RODRIGUEZ, JF ^{[1
]}

VINUELA, E ^{[1
]}

SALAS, ML ^{[1
]}

机构：

[1] UNIV AUTONOMA,CSIC,CTR BIOL MOLEC SEVERO OCHOA,E-28049 MADRID,SPAIN

来源：

GENE | 1993年 / 136卷 / 1-2期

关键词：

DNA REPLICATION; APHIDICOLIN; SEQUENCE HOMOLOGY; GENE EXPRESSION; TRANSCRIPTION INITIATION;

D O I：

10.1016/0378-1119(93)90453-A

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

The putative DNA polymerase-encoding gene of African swine fever virus has been sequenced. The gene, designated G1207R, is located in the central region of the viral genome, and encodes a protein of 1207 amino acids (aa) with a predicted M(r) of 139 835. The gene is transcribed at both early and late stages of infection into a 4.1-kb RNA. Transcription is initiated at tsp, 8 nucleotides (nt) upstream from the start codon. Open reading frame (ORF) G1207R contains four direct repeats in tandem close to the 3'-end. Each repeat consists of 12 nt, coding for the reiterated sequence, K/NPAG. The deduced aa sequence of G1207R shows significant similarity with DNA polymerases from cellular and viral origin, belonging to the alpha-like family of DNA polymerases. In particular, the G1207R protein presents a colinear arrangement of all the 3'-->5' exonuclease and polymerization highly conserved aa regions characteristic of this group of DNA-dependent DNA polymerases.

引用

页码：103 / 110

页数：8