PHOTOELECTRON IMAGING AND PHOTOELECTRON LABELING

Photoelectron imaging involves the photoejection of low-energy electrons from a specimen surface exposed to UV light. The electrons are then accelerated and focused by an electron-optics system in much the same way fluorescent light is focused in an optical microscope. Thus, photoelectron imaging is the electron-optical analog of fluorescence microscopy. In combination with photoemissive labels it serves to extend the range of studies possible by fluorescence, for example in work on cell surfaces and internal structures of cells that have been exposed by detergent extraction of membranes.