SENSITIZATION OF T-LYMPHOCYTES TO THYROGLOBULIN AND THYROPEROXIDASE IN AUTOIMMUNE THYROID-DISEASES

被引：14

作者：

AKASU, F ^{[1
]}

MORITA, T ^{[1
]}

RESETKOVA, E ^{[1
]}

YOSHIKAWA, N ^{[1
]}

CARAYON, P ^{[1
]}

VOLPE, R ^{[1
]}

机构：

[1] UNIV TORONTO,WELLESLEY HOSP,ENDOCRINOL RES LAB,160 WELLESLEY ST E,TORONTO M4Y 1J3,ONTARIO,CANADA

来源：

AUTOIMMUNITY | 1993年 / 14卷 / 04期

基金：

英国医学研究理事会;

关键词：

AUTOIMMUNE THYROID DISEASE; T-LYMPHOCYTES; THYROGLOBULIN; THYROPEROXIDASE; SENSITIZATION;

D O I：

10.3109/08916939309079227

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

To investigate T-cell sensitization to thyroid autoantigens in autoimmune thyroid discase (AITD), purified soluble human thyroglobulin (Tg) and thyroid peroxidase (TPO) were used. Peripheral blood mononuclear cells (PBMC) as well as CD8-depleted, CD4-enriched PBMC (''selected'' PBMC) from 9 patients with Graves' disease (GD), 13 Hashimoto's thyroiditis (HT) and 10 healthy subjects, were cultured for 6 days with or without varying concentrations (0.1, 1.0 and 5.0 mug/ml, respectively) of Tg or TPO and their responses were evaluated using the 3H-thymidine incorporation assay. Total PBMC as well as selected PBMC from GD and HT responded to both TPO and Tg, but normal PBMC did not. This induction was more marked in ''selected'' PBMC; on the other hand, CD8 depletion did not permit normal PBMC to respond to either antigen. However, reactivity of selected AITD PBMC to Tg differed from that of TPO. Two way analysis of variance showed that the proliferative response was significantly greater with Tg than with TPO, (again particularly notable with the ''selected'' PBMC) in both GD and HT. There was no difference between control and AITD preparations when an irrelevant (renal microsomal) antigen was employed. Taken together with our previous report that CD4 cells were induced by TPO even when cultured with CD8 cells, it is evident that suppressor CD8 cells do play a role in CD4 cells from proliferating against Tg and TPO; however their function alone or in combination with suppressor-inducer CD4 cells is partially disturbed, so that T cell sensitization to Tg and TPO can be identified in the AITD PBMC.

引用

页码：261 / 268

页数：8

共 29 条

[21]

Weetman A.P., Autologous CD8-positive cells suppress cell proliferation in response to thyroid antigen in Hashimoto's thyroiditis, Clin Immunol Immunopath, 51, pp. 303-310, (1989)

[22]

Canonica G.W., Croci R., Bagnasco M., Ferrini S., Cosulich M.E., Ferringo O., Giordano G., Deficiency of the autologous mixed lymphocyte reaction in patients with autoimmune thyroid disease, Inr Arch Allergy Appl Immunol, 73, pp. 137-140, (1984)

[23]

Protti M.P., Manfredi A.A., Straub C., Howard J.F., Contitronconi B.M., CD4+ T cell response to the human acetylcholine receptor subunit in myasthenia gravis, J Immunol, 144, pp. 1276-1281, (1990)

[24]

Yoshida H., Amino N., Yagawa K., Uemura K., Satoh M., Miyai K., Kumahara Y., Association of serum antithyroid antibody with lymphocytic infiltration of the thyroid gland: Studies of 70 autopsied cases, J Clin Endocrinol Metab, 46, pp. 459-462, (1978)

[25]

Hawkins B.R., O'Connor K.J., Dawkins R.L., Dawkins B., Rodger B., Autoantibodies in an Australian population. I. Prevalence and persistence, J Clin Lab Immunol, 2, pp. 211-215, (1979)

[26]

Hawkins B.R., Cheah P.S., Dawkins R.L., Whittingham S., Burger H.G., Patel Y., Mackey I.R., Welborn T.A., Diagnostic significance of thyroid microsomal antibodies in a randomly selected population, Lancet, 2, pp. 1057-1059, (1980)

[27]

Tanner A.R., Scott-Morgan L., Mardell R., Lloyd R.S., The incidence of occult thyroid disease associated with thyroid antibodies identified on routine autoantibody screening, Acta Endocrinol, 100, pp. 31-35, (1982)

[28]

McLachlan S.M., Atherton M.C., Nakajima Y., Napier J., Jordan R.K., Clark F., Rees-Smith B., Thyroid peroxidase and the induction of autoimmune thyroid disease, Clin Exp Immunol, 79, pp. 188-192, (1990)

[29]

Kotani T., Umeki L., Hirai K., Otaki S., Experimental murine thyroiditis induced by porcine thyroid peroxidase and its transfer by the antigen-specific T cell line, Clin Exp Immunol, 80, pp. 11-18, (1990)

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