SIMULTANEOUS EXPRESSION OF CFA/I AND CS3 COLONIZATION FACTOR ANTIGENS OF ENTEROTOXIGENIC ESCHERICHIA-COLI BY DELTA-AROC, DELTA-AROD SALMONELLA-TYPHI VACCINE STRAIN CVD-908

被引：42

作者：

GIRON, JA ^{[1
]}

XU, JG ^{[1
]}

GONZALEZ, CR ^{[1
]}

HONE, D ^{[1
]}

KAPER, JB ^{[1
]}

LEVINE, MM ^{[1
]}

机构：

[1] UNIV MARYLAND, SCH MED, CTR VACCINE DEV, DIV GEOGR MED, BALTIMORE, MD 21201 USA

来源：

VACCINE | 1995年 / 13卷 / 10期

关键词：

FIMBRIAE; PILI; DIARRHEA; ENTEROTOXIGENIC ESCHERICHIA-COLI; SALMONELLA TYPHI; COLONIZATION FACTORS; TYPHOID FEVER;

D O I：

10.1016/0264-410X(95)00003-J

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Among the known colonization factors of enterotoxigenic Escherichia coli (ETEC), CFA/I and CS3 (the common antigen in the CFA/II family of fimbrial antigens) are two of the most prevalent fimbrial antigens found in clinical isolates but are never expressed by the same wild-type strain. We manipulated the genetic determinants encoding CS3 and CFA/I fimbriae so that these two important colonization factors are expressed simultaneously in attenuated Salmonella typhi live oral vaccine strain CVD 908, including after growth in liquid medium (CFA/I is poorly expressed by wild-type ETEC in broth culture), The recombinant fimbrial structures produced by CVD 908 ave morphologically indistinguishable from the CS3 fibrillae and CFA/I rod-like fimbriae produced by ETEC, and are recognized by monospecific CS3 and CFA/I antibodies, This prototype construct may prove useful in investigating the live vector approach to immunoprophylaxis of ETEC diarrheal disease.

引用

页码：939 / 946

页数：8

共 59 条

[41] EXPRESSION OF CFA/I FIMBRIAE IS POSITIVELY REGULATED
SAVELKOUL, PHM
WILLSHAW, GA
MCCONNELL, MM
SMITH, HR
HAMERS, AM
VANDERZEIJST, BAM
GAASTRA, W
[J]. MICROBIAL PATHOGENESIS, 1990, 8 (02) : 91 - 99
[42] MAPPING OF A PLASMID, CODING FOR COLONIZATION FACTOR ANTIGEN-I AND HEAT-STABLE ENTERO-TOXIN PRODUCTION, ISOLATED FROM AN ENTERO-TOXIGENIC STRAIN OF ESCHERICHIA-COLI
SMITH, HR
WILLSHAW, GA
ROWE, B
[J]. JOURNAL OF BACTERIOLOGY, 1982, 149 (01) : 264 - 275
[43] STUDIES ON ESCHERICHIA COLI ENTEROTOXIN
SMITH, HW
HALLS, S
[J]. JOURNAL OF PATHOLOGY AND BACTERIOLOGY, 1967, 93 (02): : 531 - &
[44] SMYTH CJ, 1982, J GEN MICROBIOL, V128, P2081
[45] SNYDER JD, 1982, B WORLD HEALTH ORGAN, V60, P605
[46] DEVELOPMENT OF ORAL VACCINES AGAINST ENTEROTOXINOGENIC ESCHERICHIA-COLI DIARRHEA
SVENNERHOLM, AM
HOLMGREN, J
SACK, DA
[J]. VACCINE, 1989, 7 (03) : 196 - 198
[47] PURIFICATION, MORPHOLOGY, AND GENETICS OF A NEW FIMBRIAL PUTATIVE COLONIZATION FACTOR OF ENTEROTOXIGENIC ESCHERICHIA-COLI O159-H4
TACKET, CO
MANEVAL, DR
LEVINE, MM
[J]. INFECTION AND IMMUNITY, 1987, 55 (05) : 1063 - 1069
[48] CLINICAL ACCEPTABILITY AND IMMUNOGENICITY OF CVD 908 SALMONELLA-TYPHI VACCINE STRAIN
TACKET, CO
HONE, DM
LOSONSKY, GA
GUERS, L
EDELMAN, R
LEVINE, MM
[J]. VACCINE, 1992, 10 (07) : 443 - 446
[49] USE OF PHOA GENE FUSIONS TO IDENTIFY A PILUS COLONIZATION FACTOR COORDINATELY REGULATED WITH CHOLERA-TOXIN
TAYLOR, RK
MILLER, VL
FURLONG, DB
MEKALANOS, JJ
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1987, 84 (09) : 2833 - 2837
[50] NEW FIMBRIAL ANTIGENIC TYPE (E8775) THAT MAY REPRESENT A COLONIZATION FACTOR IN ENTERO-TOXIGENIC ESCHERICHIA-COLI IN HUMANS
THOMAS, LV
CRAVIOTO, A
SCOTLAND, SM
ROWE, B
[J]. INFECTION AND IMMUNITY, 1982, 35 (03) : 1119 - 1124

← 1 2 3 4 5 6 →