METHODOLOGY OF MICRODIALYSIS OF NEOSTRIATUM IN HEMIPARKINSONIAN NONHUMAN-PRIMATES

被引:22
作者
WANG, J
SKIRBOLL, S
AIGNER, TG
SAUNDERS, RC
HSIAO, J
BANKIEWICZ, KS
机构
[1] NINCDS, SURG NEUROL BRANCH, BLDG 10, ROOM 5D37, BETHESDA, MD 20892 USA
[2] HOWARD HUGHES MED INST, RES SCHOLAR PROGRAM, COCONUT GROVE, FL 33133 USA
[3] NIMH, NEUROPSYCHOL BRANCH, BETHESDA, MD 20892 USA
[4] NIMH, NEUROPHARMACOL CLIN, BETHESDA, MD 20892 USA
关键词
D O I
10.1016/0014-4886(90)90028-Q
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
In vivo biochemical microdialysis in primate brain would greatly expand our understanding of functional neuronal systems. This work describes our efforts to establish a microdialysis system in primate brain. Seven anesthetized rhesus monkeys underwent magnetic resonance imaging (MRI) with the head fixed in a compatible stereotaxic frame. This allowed stereotaxic localization of the caudate nucleus and putamen. Guide cannulae were implanted and fixed to the skull. Microdialysis probes made from polyethylene and fused silica were inserted into the caudate and putamen through the guide cannulae and perfused at the rate of 1.3 μl/min. The putamens were approached horizontally, while the caudate nuclei were reached via a 30°-45° angle from the vertical. Postdialysis MRI and histologic evaluation proved that all probes accurately arrived at the predetermined region. Our data show that MRI guided stereotaxis allows accurate placement of dialysis probes and that implantation of guide cannulae is a reliable and convenient way to perform repeated brain microdialysis procedures. © 1990.
引用
收藏
页码:181 / 186
页数:6
相关论文
共 23 条
[1]  
BAKAY RAE, 1988, PROG BRAIN RES, V78, P463
[2]  
BANKIEWICZ KS, 1986, LIFE SCI, V39, P7, DOI 10.1016/0024-3205(86)90431-5
[3]  
BANKIEWICZ KS, UNPUB DOPAMINIE TYRO
[4]  
BANKIEWICZ KS, 1989, AM ASS NEUR SURG ABS, P216
[6]  
COFFEY RJ, 1987, APPL NEUROPHYSIOL, V50, P81
[7]  
Feger J, 1975, ADV NEUROL, P35
[8]  
Gergen J.A., 1962, STEREOTAXIC ATLAS SQ, DOI DOI 10.5962/BHL.TITLE.114088
[9]   The structure and functions of the cerebellum examined by a new method. [J].
Horsley, V ;
Clarke, RH .
BRAIN, 1908, 31 :45-124
[10]  
ILINSKY IA, 1982, APPL NEUROPHYSIOL, V45, P563