LONG-TERM EXPRESSION OF HUMAN ADENOSINE-DEAMINASE IN VASCULAR SMOOTH-MUSCLE CELLS OF RATS - A MODEL FOR GENE-THERAPY

被引：109

作者：

LYNCH, CM

CLOWES, MM

OSBORNE, WRA

CLOWES, AW

MILLER, AD

机构：

[1] UNIV WASHINGTON,DEPT SURG,SEATTLE,WA 98195

[2] UNIV WASHINGTON,DEPT PEDIAT,SEATTLE,WA 98195

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1992年 / 89卷 / 03期

关键词：

RETROVIRAL VECTORS; BETA-GALACTOSIDASE; NEOMYCIN PHOSPHOTRANSFERASE; GENE TRANSFER;

D O I：

10.1073/pnas.89.3.1138

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Gene transfer into vascular smooth muscle cells in animals was examined by using recombinant retroviral vectors containing an Escherichia coli beta-galactosidase gene or a human adenosine deaminase (adenosine aminohydrolase, EC 3.5.4.4) gene. Direct gene transfer by infusion of virus into rat carotid arteries was not observed. However, gene transfer by infection of smooth muscle cells in culture and seeding of the transduced cells onto arteries that had been denuded of endothelial cells was successful. Potentially therapeutic levels of human adenosine deaminase activity were detected over 6 months of observation, indicating the utility of vascular smooth muscle cells for gene therapy in humans.

引用

页码：1138 / 1142

页数：5

共 30 条

[1] INTERNAL INITIATION OF TRANSLATION IN RETROVIRAL VECTORS CARRYING PICORNAVIRUS-5' NONTRANSLATED REGIONS [J].