LONG-TERM EXPRESSION OF HUMAN ADENOSINE-DEAMINASE IN VASCULAR SMOOTH-MUSCLE CELLS OF RATS - A MODEL FOR GENE-THERAPY

被引：109

作者：

LYNCH, CM

CLOWES, MM

OSBORNE, WRA

CLOWES, AW

MILLER, AD

机构：

[1] UNIV WASHINGTON,DEPT SURG,SEATTLE,WA 98195

[2] UNIV WASHINGTON,DEPT PEDIAT,SEATTLE,WA 98195

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1992年 / 89卷 / 03期

关键词：

RETROVIRAL VECTORS; BETA-GALACTOSIDASE; NEOMYCIN PHOSPHOTRANSFERASE; GENE TRANSFER;

D O I：

10.1073/pnas.89.3.1138

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Gene transfer into vascular smooth muscle cells in animals was examined by using recombinant retroviral vectors containing an Escherichia coli beta-galactosidase gene or a human adenosine deaminase (adenosine aminohydrolase, EC 3.5.4.4) gene. Direct gene transfer by infusion of virus into rat carotid arteries was not observed. However, gene transfer by infection of smooth muscle cells in culture and seeding of the transduced cells onto arteries that had been denuded of endothelial cells was successful. Potentially therapeutic levels of human adenosine deaminase activity were detected over 6 months of observation, indicating the utility of vascular smooth muscle cells for gene therapy in humans.

引用

收藏

页码：1138 / 1142

页数：5

相关论文

共 30 条

[21] GENETICALLY MODIFIED SKIN FIBROBLASTS PERSIST LONG AFTER TRANSPLANTATION BUT GRADUALLY INACTIVATE INTRODUCED GENES [J].

PALMER, TD ;

ROSMAN, GJ ;

OSBORNE, WRA ;

MILLER, AD .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1991, 88 (04) :1330-1334

[22] EFFICIENT RETROVIRUS-MEDIATED TRANSFER AND EXPRESSION OF A HUMAN ADENOSINE-DEAMINASE GENE IN DIPLOID SKIN FIBROBLASTS FROM AN ADENOSINE DEAMINASE-DEFICIENT HUMAN [J].

PALMER, TD ;

HOCK, RA ;

OSBORNE, WRA ;

MILLER, AD .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1987, 84 (04) :1055-1059

[23]

PEARSON B, 1963, LAB INVEST, V12, P1249

[24] INTERNAL INITIATION OF TRANSLATION OF EUKARYOTIC MESSENGER-RNA DIRECTED BY A SEQUENCE DERIVED FROM POLIOVIRUS RNA [J].

PELLETIER, J ;

SONENBERG, N .

NATURE, 1988, 334 (6180) :320-325

[25] INTRODUCTION OF VASCULAR SMOOTH-MUSCLE CELLS EXPRESSING RECOMBINANT GENES INVIVO [J].

PLAUTZ, G ;

NABEL, EG ;

NABEL, GJ .

CIRCULATION, 1991, 83 (02) :578-583

[26] GENE-TRANSFER INTO HUMANS - IMMUNOTHERAPY OF PATIENTS WITH ADVANCED MELANOMA, USING TUMOR-INFILTRATING LYMPHOCYTES MODIFIED BY RETROVIRAL GENE TRANSDUCTION [J].

ROSENBERG, SA ;

AEBERSOLD, P ;

CORNETTA, K ;

KASID, A ;

MORGAN, RA ;

MOEN, R ;

KARSON, EM ;

LOTZE, MT ;

YANG, JC ;

TOPALIAN, SL ;

MERINO, MJ ;

CULVER, K ;

MILLER, AD ;

BLAESE, RM ;

ANDERSON, WF .

NEW ENGLAND JOURNAL OF MEDICINE, 1990, 323 (09) :570-578

[27] USE OF A RECOMBINANT RETROVIRUS TO STUDY POSTIMPLANTATION CELL LINEAGE IN MOUSE EMBRYOS [J].

SANES, JR ;

RUBENSTEIN, JLR ;

NICOLAS, JF .

EMBO JOURNAL, 1986, 5 (12) :3133-3142

[28]

TSUKADA T, 1987, AM J PATHOL, V126, P51

[29] CONSTRUCTION AND ISOLATION OF A TRANSMISSIBLE RETROVIRUS CONTAINING THE SRC GENE OF HARVEY MURINE SARCOMA-VIRUS AND THE THYMIDINE KINASE GENE OF HERPES-SIMPLEX VIRUS TYPE-1 [J].

WEI, CM ;

GIBSON, M ;

SPEAR, PG ;

SCOLNICK, EM .

JOURNAL OF VIROLOGY, 1981, 39 (03) :935-944

[30] IMPLANTATION OF VASCULAR GRAFTS LINED WITH GENETICALLY MODIFIED ENDOTHELIAL-CELLS [J].

WILSON, JM ;

BIRINYI, LK ;

SALOMON, RN ;

LIBBY, P ;

CALLOW, AD ;

MULLIGAN, RC .

SCIENCE, 1989, 244 (4910) :1344-1346