DNA SEPARATIONS BY SLAB GEL AND CAPILLARY ELECTROPHORESIS - THEORY AND PRACTICE

被引:50
作者
BARRON, AE
BLANCH, HW
机构
[1] Department of Chemical Engineering, University of California, Berkeley
来源
SEPARATION AND PURIFICATION METHODS | 1995年 / 24卷 / 01期
关键词
D O I
10.1080/03602549508014343
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
In this review we evaluate existing experimental methods for the electrophoretic size separation of nucleic acids, including both slab gel electrophoresis and capillary electrophoresis (CE). First, we briefly discuss some important applications of DNA electrophoresis in molecular biology. We then focus on the theory and the practice of DNA separations by slab gel electrophoresis and CE, emphasizing the different practical limitations of the two techniques. In particular, we review new developments in CE, a technique which is evolving rapidly.
引用
收藏
页码:1 / 118
页数:118
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