A BACTERIOPHAGE-LAMBDA VECTOR FOR CLONING WITH BAMHI AND SAU3A

被引：71

作者：

MIZUSAWA, S ^{[1
]}

WARD, DF ^{[1
]}

机构：

[1] NCI, MOLEC BIOL LAB, BETHESDA, MD 20205 USA

来源：

GENE | 1982年 / 20卷 / 03期

关键词：

D O I：

10.1016/0378-1119(82)90200-1

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

A phage .lambda. cloning vector was constructed which contains a single site for the restriction endonuclease BamHI. Since Sau3A and BglII produce the same cohesive ends as BamHI, this vector can also be used to clone DNA fragments generated with either of these enzymes. This vector was used to construct an Escherichia coli library using partial digestion with Sau3A. This vector will be most useful for applications requiring genetic analysis of cloned E. coli genes.

引用

页码：317 / 322

页数：6

共 14 条

[1] ISOLATION OF HIGH MOLECULAR WEIGHT DNA FROM HEMOPHILUS INFLUENZAE
BERNS, KI
THOMAS, CA
[J]. JOURNAL OF MOLECULAR BIOLOGY, 1965, 11 (03) : 476 - &
[2] DANIELS DL, 1982, GENETIC MAPS, V2, P1
[3] Enquist L, 1979, Methods Enzymol, V68, P281
[4] ENQUIST LW, 1976, J MOL BIOL, V111, P97
[5] INTEGRATION-NEGATIVE MUTANTS OF BACTERIOPHAGE LAMBDA
GOTTESMAN, ME
YARMOLINSKY, MB
[J]. JOURNAL OF MOLECULAR BIOLOGY, 1968, 31 (03) : 487 - +
[6] PHAGE LAMBDA RECEPTOR CHROMOSOMES FOR DNA FRAGMENTS MADE WITH RESTRICTION ENDONUCLEASE-I OF BACILLUS-AMYLOLIQUEFACIENS-H
KLEIN, B
MURRAY, K
[J]. JOURNAL OF MOLECULAR BIOLOGY, 1979, 133 (02) : 289 - 294
[7] PHAGE LAMBDA-RECEPTOR CHROMOSOMES FOR DNA FRAGMENTS MADE WITH RESTRICTION ENDONUCLEASE-3 OF HEMOPHILUS-INFLUENZAE AND RESTRICTION ENDONUCLEASE-1 OF ESCHERICHIA-COLI
MURRAY, K
MURRAY, NE
[J]. JOURNAL OF MOLECULAR BIOLOGY, 1975, 98 (03) : 551 - 564
[8] LAMBDOID PHAGES THAT SIMPLIFY RECOVERY OF INVITRO RECOMBINANTS
MURRAY, NE
BRAMMAR, WJ
MURRAY, K
[J]. MOLECULAR & GENERAL GENETICS, 1977, 150 (01): : 53 - 61
[9] MANIPULATION OF RESTRICTION TARGETS IN PHAGE LAMBDA TO FORM RECEPTOR CHROMOSOMES FOR DNA FRAGMENTS
MURRAY, NE
MURRAY, K
[J]. NATURE, 1974, 251 (5475) : 476 - 481
[10] DNA TARGETS FOR ESCHERICHIA-COLI K RESTRICTION SYSTEM ANALYZED GENETICALLY IN RECOMBINANTS BETWEEN PHAGES PHI80 AND LAMBDA
MURRAY, NE
DERITIS, PM
FOSTER, LA
[J]. MOLECULAR AND GENERAL GENETICS, 1973, 120 (03): : 261 - 281

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