DETECTION OF BORNA-DISEASE VIRUS-RNA IN NATURALLY INFECTED ANIMALS BY A NESTED POLYMERASE CHAIN-REACTION

被引:36
作者
ZIMMERMANN, W
DURRWALD, R
LUDWIG, H
机构
[1] Institut für Virologie, Freie Universität Berlin
关键词
BORNA DISEASE VIRUS (BDV); REVERSE TRANSCRIPTASE PCR; RNA EXTRACTION;
D O I
10.1016/0166-0934(94)90098-1
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Borna disease virus in naturally infected horses, a donkey and sheep was detected for the first time by amplification of viral RNA using PCR. In contrast to a control group of healthy horses, brain tissue was positive by this assay in all animals with neurological symptoms. The use of a second round of PCR with nested primers following Southern hybridization confirmed the specificity and increased the sensitivity of the test. Comparison with conventional methods recommends this technique for monitoring of BDV infections at a molecular level.
引用
收藏
页码:133 / 143
页数:11
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