PHYSICAL MAP OF THE EXTREMELY THERMOPHILIC BACTERIUM THERMUS-THERMOPHILUS HB27 CHROMOSOME

被引：21

作者：

TABATA, K ^{[1
]}

KOSUGE, T ^{[1
]}

NAKAHARA, T ^{[1
]}

HOSHINO, T ^{[1
]}

机构：

[1] UNIV TSUKUBA,INST APPL BIOCHEM,TSUKUBA,IBARAKI 305,JAPAN

来源：

FEBS LETTERS | 1993年 / 331卷 / 1-2期

关键词：

PHYSICAL MAP; EXTREME THERMOPHILE; THERMUS-THERMOPHILUS;

D O I：

10.1016/0014-5793(93)80301-A

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The physical map of the chromosome of Thermus thermophilus HB27 was constructed using three restriction enzymes; EcoRI, SspI and MunI by applying pulsed-field gel electrophoresis techniques. Although the genome size of 1.82 Mb was almost the same as that (1.74 Mb) reported for T. thermophilus HB8[Borges, K.M., and P.L. Bergquist. (1993) J. Bacteriol. 175,103-110], the MunI cleavage maps were different. A 240 kb plasmid was detected in HB27, and its physical map was also constructed. In addition, several genes were located on the chromosomal physical map.

引用

页码：81 / 85

页数：5