REPLICATION OF MAIZE STREAK VIRUS MUTANTS IN MAIZE PROTOPLASTS - EVIDENCE FOR A MOVEMENT PROTEIN

被引:71
作者
BOULTON, MI
PALLAGHY, CK
CHATANI, M
MACFARLANE, S
DAVIES, JW
机构
[1] Virus Research Department, John Innes Institute, John Innes Centre for Plant Science Research, Norwich, NR4 7UH, Colney Lane
[2] Deparlment of Botany, La Trobe Universtty, Bundoora, VIC
[3] Nippon Oil Company, Yamaguchi 744, 766 Higashi-Toyoi, Ku-damatsu-Shi
关键词
D O I
10.1006/viro.1993.1010
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
We have used protoplasts derived from a maize (Black Mexican Sweet) suspension culture to study the replication of maize streak virus (MSV) wild-type (wt) and mutant DNA genomes. Following inoculation with plasmids containing multimeric copies of MSV, both single-stranded (ss) and double-stranded MSV DNA forms were produced, in proportions relative to those seen in infected plants. The immunodetection of coat protein (PV2) and geminate particles confirmed that the protoplasts were able to support the entire multiplication cycle of MSV and therefore were suitable for the study of MSV gene function. Inoculation of protoplasts with VI gene mutants which are unable to produce systemic infections in plants resulted in DNA replication and encapsidation indistinguishable from that obtained with wt constructs implicating the protein in virus movement. Computer analysis of the V1 protein (PV1) indicated a potential trans-membrane or membrane-embedded domain. Following inoculation of protoplasts with V2 mutants, ssDNA was not detected; the inability of these mutants to spread in plants may be due to a lack of ssDNA, PV2, or a combination of the two. The requirements for systemic infection of MSV are discussed. © 1993 Academic Press. All rights reserved.
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页码:85 / 93
页数:9
相关论文
共 43 条
  • [11] THE NUCLEOTIDE-SEQUENCE AND GENOME STRUCTURE OF THE GEMINIVIRUS MISCANTHUS STREAK VIRUS
    CHATANI, M
    MATSUMOTO, Y
    MIZUTA, H
    IKEGAMI, M
    BOULTON, MI
    DAVIES, JW
    [J]. JOURNAL OF GENERAL VIROLOGY, 1991, 72 : 2325 - 2331
  • [12] PREDICTION OF PROTEIN CONFORMATION
    CHOU, PY
    FASMAN, GD
    [J]. BIOCHEMISTRY, 1974, 13 (02) : 222 - 245
  • [13] TRANSCRIPTION OF CAULIFLOWER MOSAIC-VIRUS DNA - DETECTION OF TRANSCRIPTS, PROPERTIES, AND LOCATION OF THE GENE ENCODING THE VIRUS INCLUSION BODY PROTEIN
    COVEY, SN
    HULL, R
    [J]. VIROLOGY, 1981, 111 (02) : 463 - 474
  • [14] A COMPREHENSIVE SET OF SEQUENCE-ANALYSIS PROGRAMS FOR THE VAX
    DEVEREUX, J
    HAEBERLI, P
    SMITHIES, O
    [J]. NUCLEIC ACIDS RESEARCH, 1984, 12 (01) : 387 - 395
  • [15] THE NUCLEOTIDE-SEQUENCE OF A GEMINIVIRUS FROM DIGITARIA-SANGUINALIS
    DONSON, J
    ACCOTTO, GP
    BOULTON, MI
    MULLINEAUX, PM
    DAVIES, JW
    [J]. VIROLOGY, 1987, 161 (01) : 160 - 169
  • [16] DONSON J, 1984, EMBO J, V3, P3069, DOI 10.1002/j.1460-2075.1984.tb02260.x
  • [17] SIZE REVERSION OF AFRICAN CASSAVA MOSAIC-VIRUS COAT PROTEIN GENE DELETION MUTANTS DURING INFECTION OF NICOTIANA-BENTHAMIANA
    ETESSAMI, P
    WATTS, J
    STANLEY, J
    [J]. JOURNAL OF GENERAL VIROLOGY, 1989, 70 : 277 - 289
  • [18] Francki RIB, 1991, ARCH VIROL S, V2
  • [19] GENETIC-ANALYSIS OF TOMATO GOLDEN MOSAIC-VIRUS - THE COAT PROTEIN IS NOT REQUIRED FOR SYSTEMIC SPREAD OR SYMPTOM DEVELOPMENT
    GARDINER, WE
    SUNTER, G
    BRAND, L
    ELMER, JS
    ROGERS, SG
    BISARO, DM
    [J]. EMBO JOURNAL, 1988, 7 (04) : 899 - 904
  • [20] AGROBACTERIUM-MEDIATED DELIVERY OF INFECTIOUS MAIZE STREAK VIRUS INTO MAIZE PLANTS
    GRIMSLEY, N
    HOHN, T
    DAVIES, JW
    HOHN, B
    [J]. NATURE, 1987, 325 (6100) : 177 - 179