REGIOSELECTIVE ANALYSIS OF TRIACYLGLYCEROLS BY LIPASE HYDROLYSIS

A modified procedure for the regiospecific analysis of triacylglycerols (TAG) with a 1,3-specific lipase is described. After partial lipase hydrolysis of the triacylglycerol, th released free fatty acids (FFA) and 1,2(2,3)-diacylglycerols (DAC) were isolated by thin-layer chromatography (TLC) and converted to fatty acid methyl esters (FAME). The FAME were analyzed by gas-liquid chromatography (GLC). The 1,3-specific lipases used in this study included supported preparations from strains of Mucor miehei and Rhizopus oryzae. The method also was applied to the regiospecific analyses of tung nut and Chinese melon seed oil triacyglycerols, both of which contain high proportions of alpha-elaeostearic acid, The TAG composition of the oils was substantiated in parallel analysis of the oils by high-performance liquid chromatography with chemical ionization mass spectrometric detection of intact TAC.