ISOLATION AND SEQUENCING OF A CDNA-ENCODING THE HYPOXANTHINE-GUANINE PHOSPHORIBOSYLTRANSFERASE FROM TOXOPLASMA-GONDII

被引：8

作者：

VASANTHAKUMAR, G

VANGINKEL, S

PARISH, G

机构：

[1] Structural and Molecular Biology Group, Pharmaceutical Division, Southern Research Institute, Birmingham

来源：

GENE | 1994年 / 147卷 / 01期

关键词：

RECOMBINANT DNA; MOLECULAR CLONING; LAMBDA-ZAPII; COMPLEMENTATION; PATHOGEN; PURINE PATHWAY; HGPRT-DEFICIENT ESCHERICHIA COLI;

D O I：

10.1016/0378-1119(94)90058-2

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

A hypoxanthine-guanine phosphoribosyltransferase-encoding gene (HGPRT) from Toxoplasma gondii has been isolated and sequenced. The identity of the enzyme was determined by sequence comparison and complementation in a mutant Escherichia coli.

引用

页码：153 / 154

页数：2

共 15 条

[11] TOXOPLASMIC ENCEPHALITIS IN PATIENTS WITH ACQUIRED IMMUNE-DEFICIENCY SYNDROME [J].

LUFT, BJ ;

BROOKS, RG ;

CONLEY, FK ;

MCCABE, RE ;

REMINGTON, JS .

JAMA-JOURNAL OF THE AMERICAN MEDICAL ASSOCIATION, 1984, 252 (07) :913-917

[12] NUCLEOTIDE-SEQUENCE OF A GENE, HPT, FOR HYPOXANTHINE PHOSPHORIBOSYLTRANSFERASE FROM VIBRIO-HARVEYI [J].

SHOWALTER, RE ;

SILVERMAN, MR .

NUCLEIC ACIDS RESEARCH, 1990, 18 (15) :4621-4621

[13] HYPOXANTHINE TRANSPORT AND METABOLISM IN THE CENTRAL NERVOUS-SYSTEM [J].

SPECTOR, R .

JOURNAL OF NEUROCHEMISTRY, 1988, 50 (03) :969-978

[14] USE OF THE HPRT GENE AND THE HAT SELECTION TECHNIQUE IN DNA-MEDIATED TRANSFORMATION OF MAMMALIAN-CELLS - 1ST STEPS TOWARD DEVELOPING HYBRIDOMA TECHNIQUES AND GENE-THERAPY [J].

SZYBALSKI, W .

BIOESSAYS, 1992, 14 (07) :495-500

[15] CLONING AND EXPRESSION IN ESCHERICHIA-COLI OF A HYPOXANTHINE-GUANINE PHOSPHORIBOSYLTRANSFERASE-ENCODING CDNA FROM PLASMODIUM-FALCIPARUM [J].

VASANTHAKUMAR, G ;

DAVIS, RL ;

SULLIVAN, MA ;

DONAHUE, JP .

GENE, 1990, 91 (01) :63-69

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