AN AMPEROMETRIC BIENZYME ELECTRODE FOR ACETALDEHYDE DETECTION

被引：29

作者：

NOGUER, T ^{[1
]}

MARTY, JL ^{[1
]}

机构：

[1] UNIV PERPIGNAN,ETUD & RECH APPL PLURIDISCIPLINAIRES GRP,CNRS,URA 461,F-66860 PERPIGNAN,FRANCE

来源：

ENZYME AND MICROBIAL TECHNOLOGY | 1995年 / 17卷 / 05期

关键词：

ACETALDEHYDE; AMPEROMETRY; BIENZYME ELECTRODE;

D O I：

10.1016/0141-0229(94)00068-3

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

An electrochemical sensor with soluble aldehyde dehydrogenase (EC 1.2.1.5) and diaphorase (EC 1.8.1.4) allowed the detection of acetaldehyde in a linear range from 1 to 500 mu M. The experimental conditions (0.1 M phosphate buffer, pH 7.5) were chosen to take into account the characteristics of the two enzymes. The NADH formed in the oxidation of acetaldehyde was enzymatically oxidized by diaphorase using hexacyanoferrate (III), and the hexacyanoferrate (II) produced was electrochemically oxidized at a potential of 81 mV applied between two platinum electrodes. Validation of the assay was made on wine, beer, and cider by comparison with a standard spectrophotometric method.

引用

页码：453 / 456

页数：4

共 15 条

[1]

BLACK S, 1974, METHOD ENZYMOL, V1, P508

[2] AMPEROMETRIC BIENZYME ELECTRODE FOR L-CARNITINE [J].

COMTAT, M ;

GALY, M ;

GOULAS, P ;

SOUPPE, J .

ANALYTICA CHIMICA ACTA, 1988, 208 (1-2) :295-300

[3] AN ENZYMATIC ASSAY FOR THE DETERMINATION OF ACETALDEHYDE IN BEERS [J].

DELCOUR, JA ;

CAERS, JM ;

DONDEYNE, P ;

DELVAUX, F ;

ROBBERECHTS, E .

JOURNAL OF THE INSTITUTE OF BREWING, 1982, 88 (06) :384-386

[4] BIENZYME AMPEROMETRIC LACTATE-SPECIFIC ELECTRODE [J].

DURLIAT, H ;

CAUSSERAND, C ;

COMTAT, M .

ANALYTICA CHIMICA ACTA, 1990, 231 (02) :309-311

[5]

HEINTZE K, 1970, Zeitschrift fuer Lebensmittel-Untersuchung und -Forschung, V142, P40, DOI 10.1007/BF01454609

[6]

Horwitz W., 1975, OFFICIAL METHODS ANA, P1094

[7]

JAULMES P, 1935, ANN FALSIF FRAUDES, V28, P325

[8]

Joyeux A., 1979, Annales des Falsifications et de l'Expertise Chimique, V72, P321

[9]

LUNDQUIST F, 1974, METHOD ENZYMAT AN, V3, P1509

[10]

MARTY JL, 1992, ANAL LETT, V25, P1389

← 1 2 →