BIENZYME AMPEROMETRIC LACTATE-SPECIFIC ELECTRODE

被引:25
作者
DURLIAT, H [1 ]
CAUSSERAND, C [1 ]
COMTAT, M [1 ]
机构
[1] UNIV TOULOUSE 3,CHIM PHYS & ELECTROCHIM LAB,CNRS,URA 192,GENIE CHIM LAB,118 ROUTE,F-31062 TOULOUSE,FRANCE
关键词
D O I
10.1016/S0003-2670(00)86432-6
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
The electrode, based on a lactate dehydrogenase and a diaphorase, permits the assay of l-lactate in the concentration range 0.2-8 mM with a response time of about 40 s. Both the enzymes are commercially available. The amperometric detection of hexacyanoferrate(II) at a platinum electrode is done at 0.3 V (vs. SCE) instead of 0.8 V as in the detection of NADH, improving the selectivity of the sensor. © 1990.
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页码:309 / 311
页数:3
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