A RAPID POLYMERASE CHAIN-REACTION METHOD FOR IDENTIFYING FIXED SPECIMENS

被引：38

作者：

GILL, P

KIMPTON, CP

SULLIVAN, K

机构：

[1] Central Research and Support Establishment, Reading, Aldermaston

来源：

ELECTROPHORESIS | 1992年 / 13卷 / 03期

关键词：

D O I：

10.1002/elps.1150130135

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

DNA profiling analysis of preserved tissue samples was achieved by using an extraction method which incorporated the use of a chelating resin "Chelex" to chelate inhibitory polyvalent metal ions. An automated sequencer was used to estimate sizes of low molecular weight trimeric and tetrameric microsatellites labelled with fluorescent dyes. The entire analysis can be completed within 48h. Probabilities of chance association using two microsatellites were calculated as ranging between 10(-2) and 10(-4). The methods described have considerable potential for use in routine genetic analysis of preserved samples.

引用

页码：173 / 175

页数：3

共 13 条

[1] RAPID TYPING OF TANDEMLY REPEATED HYPERVARIABLE LOCI BY THE POLYMERASE CHAIN-REACTION - APPLICATION TO THE APOLIPOPROTEIN-B 3' HYPERVARIABLE REGION [J].