THE ROLE OF THE ENDOTHELIUM ON ENHANCED CONTRACTILE RESPONSE OF NON-INSULIN-DEPENDENT DIABETIC RAT AORTAE - EFFECTS OF INSULIN-TREATMENT

被引:11
作者
KARASU, C
ALTAN, VM
机构
[1] Department of Pharmacology, Faculty of Pharmacy, Ankara University, 06100 Tandogan, Ankara
来源
GENERAL PHARMACOLOGY | 1994年 / 25卷 / 04期
关键词
D O I
10.1016/0306-3623(94)90262-3
中图分类号
R9 [药学];
学科分类号
1007 ;
摘要
1. The role of the endothelium on the constrictor effect of noradrenaline (NA), and endothelium-dependent and endothelium-independent vasodilator effects of different agonists were determined in rings of aortae obtained from nondiabetic (control), non-insulin-dependent (NID-) diabetic and NID-diabetic rats treated with insulin. 2. The NA-induced contractions are markedly increased with no change in agonist potency (pD(2) value) in aortae with intact endothelium obtained from NID-diabetic rats when compared to those from age-matched controls. Mechanical removal of the endothelium resulted in a significant increase in maximum contractile response of control aortae but not NID-diabetic aortae to NA relative to presence of endothelium. No significant difference was observed between the contractile responses of NID-diabetic and control aortae to NA after the removal of endothelium. 3. Endothelium-dependent relaxations elicited by acetylcholine (ACh), histamine, ATP and insulin strongly depressed in NID-diabetic aortae but the relaxations stimulated by sodium nitroprusside (SNP) did not change when compared to corresponding controls. There was no significant changes in the pD2 values calculated by agonist-induced relaxations of aortae from either untreated or insulin-treated NID-diabetic rats compared with controls. 4. NID-diabetes-induced alterations on the reactivity of aortae were significantly restored by in vivo insulin treatment. 5. These data indicate that impaired endothelium-dependent vasodilatory responses of aortae from NID-diabetic rats results in increased NA-induced contractions. It is possible that, impaired endothelium-dependent vasodilatory modulation of vasoconstriction may play a important role in development of vasospasm and hypertension in NID-diabetics.
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页码:795 / 802
页数:8
相关论文
共 42 条
[31]   MECHANISM OF GLUCOSE-INDUCED (NA+, K+)-ATPASE INHIBITION IN AORTIC-WALL OF RABBITS [J].
SIMMONS, DA ;
WINEGRAD, AI .
DIABETOLOGIA, 1989, 32 (07) :402-408
[32]  
SIMMONS DA, 1992, DIABETOLOGIA, V35, P725
[33]   ATHEROSCLEROSIS IN DIABETES - THE ROLE OF HYPERINSULINEMIA [J].
STOLAR, MW .
METABOLISM-CLINICAL AND EXPERIMENTAL, 1988, 37 (02) :1-9
[34]   ELEVATED PLASMA ENDOTHELIN IN PATIENTS WITH DIABETES-MELLITUS [J].
TAKAHASHI, K ;
GHATEI, MA ;
LAM, HC ;
OHALLORAN, DJ ;
BLOOM, SR .
DIABETOLOGIA, 1990, 33 (05) :306-310
[35]   CONTRACTION OF DIABETIC RABBIT AORTA CAUSED BY ENDOTHELIUM-DERIVED PGH2-TXA2 [J].
TESFAMARIAM, B ;
JAKUBOWSKI, JA ;
COHEN, RA .
AMERICAN JOURNAL OF PHYSIOLOGY, 1989, 257 (05) :H1327-H1333
[36]   THE ENDOTHELIUM - MODULATOR OF VASCULAR SMOOTH-MUSCLE TONE [J].
VANHOUTTE, PM .
NEW ENGLAND JOURNAL OF MEDICINE, 1988, 319 (08) :512-513
[37]   ISLET SECRETION IN A NEW EXPERIMENTAL-MODEL FOR NON-INSULIN-DEPENDENT DIABETES [J].
WEIR, GC ;
CLORE, ET ;
ZMACHINSKI, CJ ;
BONNERWEIR, S .
DIABETES, 1981, 30 (07) :590-595
[38]   ENHANCED VASCULAR ALPHA-ADRENERGIC NEUROEFFECTOR SYSTEM IN DIABETES - IMPORTANCE OF CALCIUM [J].
WHITE, RE ;
CARRIER, GO .
AMERICAN JOURNAL OF PHYSIOLOGY, 1988, 255 (05) :H1036-H1042
[39]   SUPERSENSITIVITY AND ENDOTHELIUM DEPENDENCY OF HISTAMINE-INDUCED RELAXATION IN MESENTERIC-ARTERIES ISOLATED FROM DIABETIC RATS [J].
WHITE, RE ;
CARRIER, GO .
PHARMACOLOGY, 1986, 33 (01) :34-38
[40]   CHARACTERIZATION OF GLUCOSE-INDUCED INSITU PROTEIN-KINASE-C ACTIVITY IN CULTURED VASCULAR SMOOTH-MUSCLE CELLS [J].
WILLIAMS, B ;
SCHRIER, RW .
DIABETES, 1992, 41 (11) :1464-1472