KLUYVEROMYCES AS A HOST FOR HETEROLOGOUS GENE-EXPRESSION - EXPRESSION AND SECRETION OF PROCHYMOSIN

被引：142

作者：

VANDENBERG, JA ^{[1
]}

VANDERLAKEN, KJ ^{[1
]}

VANOOYEN, AJJ ^{[1
]}

RENNIERS, TCHM ^{[1
]}

RIETVELD, K ^{[1
]}

SCHAAP, A ^{[1
]}

BRAKE, AJ ^{[1
]}

BISHOP, RJ ^{[1
]}

SCHULTZ, K ^{[1
]}

MOYER, D ^{[1
]}

RICHMAN, M ^{[1
]}

SHUSTER, JR ^{[1
]}

机构：

[1] CHIRON CORP, EMERYVILLE, CA 94608 USA

来源：

BIO-TECHNOLOGY | 1990年 / 8卷 / 02期

关键词：

D O I：

10.1038/nbt0290-135

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

We have developed the yeast Kluyvero- myces lactis as a host organism for the production of the milk-clotting enzyme chymosin. In contrast to Saccharomyces cerevisiae, we found that this yeast is capable of the synthesis and secretion of fully active prochymosin. Various signal sequences could be used to efficiently direct the secretion of prochymosin in Kluyvero- myces, but not in S. cerevisiae. We conclude that the efficient synthetic and secretory capacity of this heterologous protein is a property of the yeast Kluyveromyces. These results have led to the development of a large scale production process for chymosin. © 1990 Nature Publishing Group.

引用

页码：135 / 139

页数：5

共 29 条

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