THE AUTOGRAPHA-CALIFORNICA BACULOVIRUS GENOME - EVIDENCE FOR MULTIPLE REPLICATION ORIGINS

被引：155

作者：

PEARSON, M

BJORNSON, R

PEARSON, G

ROHRMANN, G

机构：

[1] OREGON STATE UNIV,DEPT AGR CHEM,CORVALLIS,OR 97331

[2] OREGON STATE UNIV,DEPT BIOCHEM & BIOPHYS,CORVALLIS,OR 97331

来源：

SCIENCE | 1992年 / 257卷 / 5075期

关键词：

D O I：

10.1126/science.1529337

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The Autographa californica multinucleocapsid nuclear polyhedrosis virus (AcMNPV), which is used for the overexpression of eukaryotic genes and is being engineered for possible use as a viral insecticide, has a circular, supercoiled genome of approximately 128 kilobases. Despite its widespread use, little is known about the mechanism by which AcMNPV replicates. Evidence is presented in this report that AcMNPV origins of DNA replication are repeated sequences each containing several closely related imperfect palindromes that are present in six regions distributed around the genome. Although AcMNPV infection-dependent plasmid replication was initiated by a single complete palindrome, the amount of replication was substantially increased in plasmids containing six or eight palindromes.

引用

页码：1382 / 1384

页数：3

共 14 条

[11] NUCLEOTIDE-SEQUENCE OF A PORTION OF THE AUTOGRAPHA-CALIFORNICA NUCLEAR POLYHEDROSIS-VIRUS GENOME CONTAINING THE ECORI SITE-RICH REGION (HR5) AND AN OPEN READING FRAME JUST 5' OF THE P10 GENE [J].