MYOFIBRILLAR AND CYTOSKELETAL ASSEMBLY IN NEONATAL RAT CARDIAC MYOCYTES CULTURED ON LAMININ AND COLLAGEN

被引:69
作者
HILENSKI, LL [1 ]
TERRACIO, L [1 ]
BORG, TK [1 ]
机构
[1] UNIV S CAROLINA,DEPT ANAT CELL BIOL & NEUROSCI,COLUMBIA,SC 29208
关键词
MYOFIBRILS; CYTOSKELETON; EXTRACELLULAR MATRIX; LAMININ; COLLAGEN; ACTIN FILAMENTS; VINCULIN; RAT;
D O I
10.1007/BF00319047
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Neonatal rat cardiomyocytes were cultured on extracellular matrix components laminin and collagens I + III to examine effects of extracellular matrix on the assembly of cytoskeletal proteins during myofibrillogenesis. Myofibril assembly was visualized by immunofluorescence of marker proteins for myofibrils (f-actin for I bands and alpha-actinin for Z bands), focal adhesions (vinculin), and transmembrane extracellular matrix receptors (beta-1 integrin) as cells spread for various times in culture. By 4 h in culture, f-actin appeared organized into nonstriated stress-fiber-like structures while alpha-actinin, vinculin and beta-1 integrin were localized in small streaks and beads. Subsequently, striated patterns were observed sequentially in the intracellular cytoskeletal components alpha-actinin, vinculin, f-actin, and then in the transmembrane beta-1 integrin receptor. These data support an earlier model for sarcomerogenesis in which stress-fiber-like structures serve as initial scaffolds upon which alpha-actinin and then vinculin-containing costameres are assembled. This sequential and temporal assembly was the same on both laminin and collagens I + III. A quantitative difference, however, was apparent on the 2 matrices. There was an increased appearance on collagens I + III of rosettes (also called podosomes or cortical actin-containing bodies in other cells) which consisted of an f-actin core surrounded by alpha-actinin, vinculin and beta-1 integrin rims. The increased incidence of rosettes in neonatal myocytes on collagens I + III suggests that these cytoskeletal complexes are involved in recognition and interaction with extracellular matrix components.
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页码:577 / 587
页数:11
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