THYROID-HORMONE RAPIDLY INDUCES HEPATIC LDL RECEPTOR MESSENGER-RNA LEVELS IN HYPOPHYSECTOMIZED RATS

被引：53

作者：

NESS, GC ^{[1
]}

ZHAO, ZH ^{[1
]}

机构：

[1] UNIV S FLORIDA,INST BIOMOLEC SCI,TAMPA,FL 33612

来源：

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS | 1994年 / 315卷 / 01期

关键词：

THYROID HORMONE; LDL RECEPTOR; NORTHERN BLOTTING; MESSENGER-RNA HALF-LIFE; IMMUNOBLOTTING;

D O I：

10.1006/abbi.1994.1490

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The effects of hypophysectomy and thyroid hormone treatment on the expression of the hepatic LDL receptor gene was studied in young male rats. Hypophysectomy lowered levels of LDL receptor mRNA and protein. It was found that increased levels of LDL receptor mRNA could be detected 30 min after giving hypophysectomized rats an intramuscular injection of 10 mu g of L-triiodothyronine (T-3) per 100 g of body weight. This dose of T-3 also increased hepatic LDL receptor protein levels within 1 h. A T-3 dose of only 0.25 mu g per 100 g increased LDL receptor mRNA levels more than threefold. The half-life of the hepatic LDL receptor mRNA was found to be about 30 min and was unaffected by T-3. The data suggests that thyroid hormone acts physiologically to induce hepatic LDL receptor expression. This action may explain, in part, the hypocholesterolemic effect of the hormone. (C) 1994 Academic Press, Inc.

引用

收藏

页码：199 / 202

页数：4

相关论文

共 21 条

[1] PURIFICATION OF BIOLOGICALLY-ACTIVE GLOBIN MESSENGER-RNA BY CHROMATOGRAPHY ON OLIGOTHYMIDYLIC-ACID-CELLULOSE [J].

AVIV, H ;

LEDER, P .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1972, 69 (06) :1408-&

[2] LIVER-TRANSPLANTATION TO PROVIDE LOW-DENSITY-LIPOPROTEIN RECEPTORS AND LOWER PLASMA-CHOLESTEROL IN A CHILD WITH HOMOZYGOUS FAMILIAL HYPERCHOLESTEROLEMIA [J].

BILHEIMER, DW ;

GOLDSTEIN, JL ;

GRUNDY, SM ;

STARZL, TE ;

BROWN, MS .

NEW ENGLAND JOURNAL OF MEDICINE, 1984, 311 (26) :1658-1664

[3] MEVINOLIN AND COLESTIPOL STIMULATE RECEPTOR-MEDIATED CLEARANCE OF LOW-DENSITY LIPOPROTEIN FROM PLASMA IN FAMILIAL HYPERCHOLESTEROLEMIA HETEROZYGOTES [J].

BILHEIMER, DW ;

GRUNDY, SM ;

BROWN, MS ;

GOLDSTEIN, JL .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1983, 80 (13) :4124-4128

[4] A RECEPTOR-MEDIATED PATHWAY FOR CHOLESTEROL HOMEOSTASIS [J].

BROWN, MS ;

GOLDSTEIN, JL .

SCIENCE, 1986, 232 (4746) :34-47

[5]

CHOMCZYNSKI P, 1987, ANAL BIOCHEM, V162, P156, DOI 10.1016/0003-2697(87)90021-2

[6] REGULATION OF THE MEVALONATE PATHWAY [J].

GOLDSTEIN, JL ;

BROWN, MS .

NATURE, 1990, 343 (6257) :425-430

[7] INDUCTION OF A RAPIDLY RESPONSIVE HEPATIC GENE-PRODUCT BY THYROID-HORMONE REQUIRES ONGOING PROTEIN-SYNTHESIS [J].

JACOBY, DB ;

ENGLE, JA ;

TOWLE, HC .

MOLECULAR AND CELLULAR BIOLOGY, 1987, 7 (04) :1352-1357

[8]

KOVANEN PT, 1979, J BIOL CHEM, V254, P1367

[9] NUCLEOTIDE-SEQUENCE OF THE RAT LOW-DENSITY LIPOPROTEIN RECEPTOR CDNA [J].

LEE, LY ;

MOHLER, WA ;

SCHAFER, BL ;

FREUDENBERGER, JS ;

BYRNECONNOLLY, N ;

EAGER, KB ;

MOSLEY, ST ;

LEIGHTON, JK ;

THRIFT, RN ;

DAVIS, RA ;

TANAKA, RD .

NUCLEIC ACIDS RESEARCH, 1989, 17 (03) :1259-1260

[10] EFFECT OF THYROID-HORMONE ON HEPATIC CHOLESTEROL 7-ALPHA-HYDROXYLASE, LDL RECEPTOR, HMG-COA REDUCTASE, FARNESYL PYROPHOSPHATE SYNTHETASE AND APOLIPOPROTEIN-A-I MESSENGER-RNA LEVELS IN HYPOPHYSECTOMIZED RATS [J].

NESS, GC ;

PENDLETON, LC ;

LI, YC ;

CHIANG, JYL .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1990, 172 (03) :1150-1156