DETERMINATION OF NEUTRAL OLIGOSACCHARIDE FRACTIONS FROM HUMAN-MILK BY GEL-PERMEATION CHROMATOGRAPHY

被引：34

作者：

THURL, S

OFFERMANNS, J

MULLERWERNER, B

SAWATZKI, G

机构：

[1] Research Department, Milupa AG, Bahnstrasse 14-30

来源：

JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS | 1991年 / 568卷 / 02期

关键词：

D O I：

10.1016/0378-4347(91)80166-A

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A gel permeation chromatographic method for quantifying neutral oligosaccharide fractions from human milk has been developed. Oligosaccharides from monofucosyllactoses to trifucosyllacto-N-hexaoses were separated according to size on a Fractogel TSK HW 40 (S) column. Refractive index detection of monofucosyllactoses to difucosyllacto-N-tetraoses yielded a constant mass response factor of ca. 1 relative to glucose. After the addition of glucose as an internal standard, oligosaccharides were isolated from human milk by ethanol precipitation or two ultrafiltration procedures. The oligosaccharide concentrations found by the ultrafiltration procedures were significantly lower (significance level 0.05) than those determined by the ethanol precipitation procedure.

引用

页码：291 / 300

页数：10

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