HUMAN ALU SUBFAMILIES AND THEIR METHYLATION REVEALED BY BLOT HYBRIDIZATION

被引：96

作者：

SCHMID, CW ^{[1
]}

机构：

[1] UNIV CALIF DAVIS,DEPT CHEM,DAVIS,CA 95616

来源：

NUCLEIC ACIDS RESEARCH | 1991年 / 19卷 / 20期

关键词：

D O I：

10.1093/nar/19.20.5613

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

By a simple direct blot hybridization strategy, the existence of human Alu family subfamilies is confirmed. Using consensus restriction cleavage sites, individual bands can be resolved from genomic human DNA digests corresponding to three distinct Alu subfamilies. Digestion with methylation sensitive and insensitive restriction enzymes shows that the numerous CpG residues in the youngest Alu subfamilies are largely methylated in vivo, suggesting a model for the transcriptional regulation of Alu repeats.

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页码：5613 / 5617

页数：5

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