A NEUROSECRETORY GRANULE LYS-ARG CA2+-DEPENDENT ENDOPEPTIDASE PUTATIVELY INVOLVED IN PROOXYTOCIN AND PROVASOPRESSIN PROCESSING

被引：8

作者：

ROUILLE, Y ^{[1
]}

SPANG, A ^{[1
]}

CHAUVET, J ^{[1
]}

ACHER, R ^{[1
]}

机构：

[1] UNIV PARIS 06,BIOL CHEM LAB,96 BLVD RASPAIL,F-75006 PARIS,FRANCE

来源：

NEUROPEPTIDES | 1992年 / 22卷 / 04期

关键词：

D O I：

10.1016/0143-4179(92)90050-7

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

A Ca2+-dependent endopeptidase cleaving at the carboxyl side of the paired Lys-Arg residues has been found in the neurosecretory granules of the rat neurointermediate pituitary. The specificity pattern on synthetic fluorogenic substrates, the inhibitor profile, the pH optimum of 5.0 and the Ca2+-dependence are compatible with an involvement of this enzyme in the prooxytocin and the provasopressin processing within the granules. The enzymatic features of the neurohypophysial granule endopeptidase resemble those of the insulinoma granule type II endopeptidase and suggest that the same Ca2+-dependent protease or closely related enzymes could be involved in processing Lys-Arg-containing prohormones in neuroendocrine cells.

引用

页码：223 / 228

页数：6

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