INHIBITION OF CALCINEURIN BY CYCLOSPORINE-A CYCLOPHILIN REQUIRES CALCINEURIN-B

被引：53

作者：

HADDY, A ^{[1
]}

SWANSON, SKH ^{[1
]}

BORN, TL ^{[1
]}

RUSNAK, F ^{[1
]}

机构：

[1] MAYO CLIN & MAYO FDN,HEMATOL RES SECT,ROCHESTER,MN 55905

来源：

FEBS LETTERS | 1992年 / 314卷 / 01期

关键词：

CALCINEURIN; CYCLOSPORINE-A; CYCLOPHILIN; IMMUNOSUPPRESSION;

D O I：

10.1016/0014-5793(92)81456-V

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The interaction of the immunosuppressive complex cyclosporin A-cyclophilin (CsA-CyP) with the Ca2+/calmodulin-dependent protein phosphatase calcineurin is investigated using a recombinant form of the A subunit of calcineurin (rCNA). Only in the presence of purified calcineurin B (CNB) does rCNA show the response of native calcineurin, i.e. 50% inhibition of rCNA phosphatase activity at 6 nM human cyclophilin B and 0.6 muM human cyclophilin A using [P-32]casein as substrate, yet stimulation of activity with p-nitrophenyl phosphate as substrate. This study demonstrates that the B subunit is necessary to confer sensitivity of calcineurin to CsA-CyP.

引用

页码：37 / 40

页数：4

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