RAPID GAS-CHROMATOGRAPHIC METHOD FOR DETERMINATION OF CHLOROPHENOXYISOBUTYRIC ACID IN PLASMA AND URINE

被引：22

作者：

GUGLER, R ^{[1
]}

JENSEN, C ^{[1
]}

机构：

[1] UNIV BONN, DEPT MED, 53 BONN, GERMANY

来源：

JOURNAL OF CHROMATOGRAPHY | 1976年 / 117卷 / 01期

关键词：

D O I：

10.1016/S0021-9673(00)81079-1

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A rapid gas chromatographic method is described for the determination of chlorophenoxyisobutyric acid (the active metabolite of clofibrate) in human plasma and urine. The assay involves an extraction into toluene and back-extraction of the chlorophenoxyisobutyric acid and the internal standard (2-naphthoic acid) into the methylating reagent (trimethylanilinium hydroxide). Concentrations of 1 .mu.g/ml in plasma and urine can easily be measured; the precision of the method is 3.3 .+-. 0.7% for plasma and 2.7 .+-. 0.4% for urine. There is no interference from endogenous compounds or from drugs commonly prescribed together with clofibrate.

引用

页码：175 / 179

页数：5

共 20 条

[11]

KNUCHEL F, 1974, ARZNEIMITTEL-FORSCH, V24, P576

[12] DIETARY AND DRUG TREATMENT OF PRIMARY HYPERLIPOPROTEINEMIA [J].

LEVY, RI ;

LUX, SE ;

FREDRICK.DS ;

BILHEIME.DW ;

BRESLOW, JL ;

SLOAN, HR ;

STONE, NJ ;

SHULMAN, R ;

HERBERT, PN ;

KRAUSS, RM .

ANNALS OF INTERNAL MEDICINE, 1972, 77 (02) :267-+

[13] LIPID LOWERING DRUGS AND HYPERLIPIDEMIA [J].

LEVY, RI ;

RIFKIND, BM .

DRUGS, 1973, 6 (01) :12-45

[14]

OLIVER M. F., 1967, PROGR BIOCHEM PHARMACOL, V2, P315

[15] FURTHER OBSERVATIONS ON THE EFFECTS OF ATROMID AND OF ETHYL CHLOROPHENOXYISOBUTYRATE ON SERUM LIPID LEVELS [J].

OLIVER, MF .

JOURNAL OF ATHEROSCLEROSIS RESEARCH, 1963, 3 (5-6) :427-444

[16]

SEDAGHAT A, 1974, J LIPID RES, V15, P352

[17]

SEDAGHAT A, 1975, EUR J CLIN INVEST, V5, P177

[18] THE RESPONSE OF HYPERLIPOPROTEINEMIAS TO ATROMID AND ETHYL CHLOROPHENOXYISOBUTYRATE [J].

STRISOWER, EH .

JOURNAL OF ATHEROSCLEROSIS RESEARCH, 1963, 3 (5-6) :445-453

[19]

THORP JM, 1962, LANCET, V1, P1323

[20] EFFECT OF ETHYL PARA-CHLOROPHENOXYISOBUTYRATE ON RAT ADIPOSE LIPOPROTEIN LIPASE ACTIVITY [J].

TOLMAN, EL ;

TEPPERMA.HM ;

TEPPERMA.J .

AMERICAN JOURNAL OF PHYSIOLOGY, 1970, 218 (05) :1313-&

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