THE EFFECT OF X-IRRADIATION ON CELL-CYCLE PROGRESSION AND CHROMATID ABERRATIONS IN STIMULATED HUMAN-LYMPHOCYTES USING COHORT ANALYSIS STUDIES

被引:15
作者
AGHAMOHAMMADI, SZ
SAVAGE, JRK
机构
[1] Division of Cell and Molecular Biology, Medical Research Council Radiobiology Unit, Chilton, Didcot
来源
MUTATION RESEARCH | 1992年 / 268卷 / 02期
关键词
CHROMATID ABERRATIONS; MITOTIC DELAY; CELL CYCLE; EFFECT OF X-IRRADIATION ON;
D O I
10.1016/0027-5107(92)90228-T
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
Stage sensitivity for the production of chromatid-type aberrations and mitotic delay has been investigated in a stimulated human lymphocyte population, following an absorbed dose of 1.5 Gy 250 kVp X-rays. BrdU replication banding was used to obtain a fine analysis of the cell cycle and to permit cohort analysis. Fluctuations in yield with sample time were found for all aberration categories, but these could not be related simply to either the developmental stage of the cells at time of exposure, or to the time-to-run to metaphase. In general G2 and late S cells had higher aberration yields than early S and pre S cell populations. Mitotic delay and perturbation at this dose extends to all sub-phases of S and is as great, if not greater, in the earliest S cells as it is in G2.
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页码:223 / 230
页数:8
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