INVESTIGATION OF THE SUBCELLULAR LOCATION OF THE TETRAPYRROLE-BIOSYNTHESIS ENZYME COPROPORPHYRINOGEN OXIDASE IN HIGHER-PLANTS

被引：92

作者：

SMITH, AG ^{[1
]}

MARSH, O ^{[1
]}

ELDER, GH ^{[1
]}

机构：

[1] UNIV WALES COLL MED,DEPT MED BIOCHEM,CARDIFF CF4 4XN,S GLAM,WALES

来源：

BIOCHEMICAL JOURNAL | 1993年 / 292卷

关键词：

D O I：

10.1042/bj2920503

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The subcellular location of two enzymes in the biosynthetic pathway for protoporphyrin IX, coproporphyrinogen (coprogen) oxidase (EC 1.3.3.3) and protoporphyrinogen (protogen) oxidase (EC 1.3.3.4) has been investigated in etiolated pea (Pisum sativum) leaves and spadices of cuckoo-pint (Arum maculatum). Plant tissue homogenized in isotonic buffer was subjected to subcellular fractionation to prepare mitochondria and plastids essentially free of contamination by other cellular organelles. as determined by marker enzymes. Protogen oxidase activity measured fluorimetrically was reproducibly found in both mitochondria and etioplasts. In contrast, coprogen oxidase could be detected only in etioplasts, using either a coupled fluorimetric assay or a sensitive radiochemical method. The implications of these results for the synthesis of mitochondrial haem in plants is discussed.

引用

页码：503 / 508

页数：6

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