DEVELOPMENT AND VALIDATION OF A HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC METHOD FOR THE DETERMINATION OF BENZOPHENONE-3 IN RATS

被引：19

作者：

ABDELNABI, IM

KADRY, AM

DAVIS, RA

ABDELRAHMAN, MS

机构：

[1] UNIV MED & DENT NEW JERSEY,NEW JERSEY MED SCH,DEPT PHARMACOL & TOXICOL,NEWARK,NJ 07103

[2] UNIV MED & DENT NEW JERSEY,SCH HLTH RELATED PROFESS,DEPT SCI,CLIN LAB,NEWARK,NJ 07103

[3] AMER CYANAMID CO,WAYNE,NJ 07470

来源：

JOURNAL OF APPLIED TOXICOLOGY | 1992年 / 12卷 / 04期

关键词：

BENZOPHENONE-3; HPLC; BIOLOGICAL FLUID; TISSUES;

D O I：

10.1002/jat.2550120408

中图分类号：

R99 [毒物学（毒理学）];

学科分类号：

100405 ;

摘要：

A precise, accurate, selective and sensitive liquid chromatographic method for the determination of benzophenone-3 in rat biological fluids and different tissues has been developed. The minimum detection limit for benzophenone-3 was 2.0 ng ml-1 and the retention time was 6.01 min. Standard curves for benzophenone-3 were linear in a wide range of concentrations in methanol and different body fluids, ranging from 6.25 ng ml-1 to 100-mu-g ml-1. To detect benzophenone-3 in rat whole blood after oral administration, HCl hydrolysis was required. Benzophenone-3 was found to produce a peak blood concentration 1 h after administration. Free benzophenone-3 in urine represented a very small percentage during the first 12 h after administration, while a higher concentration of the glucuronide conjugate was detected in the same time period.

引用

页码：255 / 259

页数：5

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