A STUDY OF THE GENOMIC DIVERSITY OF PLASMODIUM-FALCIPARUM IN SENEGAL .2. TYPING BY THE USE OF THE POLYMERASE CHAIN-REACTION

被引：33

作者：

MERCEREAUPUIJALON, O ^{[1
]}

FANDEUR, T ^{[1
]}

BONNEFOY, S ^{[1
]}

JACQUEMOT, C ^{[1
]}

SARTHOU, JL ^{[1
]}

机构：

[1] INST PASTEUR, UNITE IMMUNOL CELLULAIRE, DAKAR, SOUTH AFRICA

来源：

ACTA TROPICA | 1991年 / 49卷 / 04期

关键词：

PLASMODIUM-FALCIPARUM; ALLELIC POLYMORPHISM; POLYMERASE CHAIN REACTION; STRAIN TYPING; MALARIA VACCINE; MEROZOITE SURFACE ANTIGENS;

D O I：

10.1016/0001-706X(91)90080-4

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

The genomic polymorphism of Plasmodium falciparum was investigated in a series of samples collected in Senegal during one transmission season. PCR analysis was performed on several genes coding for blood-stage antigens: the gene for the major merozoite surface antigen P190, the gene for the second merozoite surface antigen MSA2 and the gene coding for antigen 96tR/GBP130. In each case, several distinct forms of the genes studied were observed. Both the MAD20 and K1 allelic families of P190 genes were observed. PCR analysis of a single variable region did not differentiate each isolate. However, when the data obtained for several markers are combined, each isolate had a specific genotype. Thus, using PCR to study in parallel several loci is a useful tool to genetically type strains.

引用

页码：293 / 304

页数：12

共 29 条

[1] BIANCO AE, 1987, MOL BIOCHEM PARASIT, V23, P91, DOI 10.1016/0166-6851(87)90191-5
[2] PLASMODIUM-FALCIPARUM - MOLECULAR ANALYSIS OF A PUTATIVE PROTECTIVE ANTIGEN, THE THERMOSTABLE 96-KDA PROTEIN
BONNEFOY, S
MATTEI, D
DUBREMETZ, JF
GUILLOTTE, M
JOUIN, H
OZAKI, LS
SIBILLI, L
MERCEREAUPUIJALON, O
[J]. EXPERIMENTAL PARASITOLOGY, 1988, 65 (01) : 69 - 83
[3] PLASMODIUM-FALCIPARUM - AN INTERVENING SEQUENCE IN THE GBP-130/96 TR GENE
BONNEFOY, S
MERCEREAUPUIJALON, O
[J]. EXPERIMENTAL PARASITOLOGY, 1989, 69 (01) : 37 - 43
[4] PLASMODIUM-FALCIPARUM SURFACE-ANTIGEN P190 - PREVALENCE OF A NONREPETITIVE ALLELE IN HUMANS
CERTA, U
[J]. PARASITOLOGY TODAY, 1990, 6 (12): : 373 - 373
[5] A NATURALLY-OCCURRING GENE ENCODING THE MAJOR SURFACE-ANTIGEN PRECURSOR P190 OF PLASMODIUM-FALCIPARUM LACKS TRIPEPTIDE REPEATS
CERTA, U
ROTMANN, D
MATILE, H
REBERLISKE, R
[J]. EMBO JOURNAL, 1987, 6 (13) : 4137 - 4142
[6] PLASMODIUM-FALCIPARUM - GENE STRUCTURE AND HYDROPATHY PROFILE OF THE MAJOR MEROZOITE SURFACE-ANTIGEN (GP195) OF THE UGANDA-PALO-ALTO ISOLATE
CHANG, SP
KRAMER, KJ
YAMAGA, KM
KATO, A
CASE, SE
SIDDIQUI, WA
[J]. EXPERIMENTAL PARASITOLOGY, 1988, 67 (01) : 1 - 11
[7] GENETIC DIVERSITY OF PLASMODIUM-FALCIPARUM SHOWS GEOGRAPHICAL VARIATION
CREASEY, A
FENTON, B
WALKER, A
THAITHONG, S
OLIVEIRA, S
MUTAMBU, S
WALLIKER, D
[J]. AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE, 1990, 42 (05) : 403 - 413
[8] PROTECTIVE IMMUNIZATION OF THE SQUIRREL-MONKEY AGAINST ASEXUAL BLOOD STAGES OF PLASMODIUM-FALCIPARUM BY USE OF PARASITE PROTEIN-FRACTIONS
DUBOIS, P
DEDET, JP
FANDEUR, T
ROUSSILHON, C
JENDOUBI, M
PAUILLAC, S
MERCEREAUPUIJALON, O
DASILVA, LP
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1984, 81 (01): : 229 - 232
[9] INVIVO AND INVITRO DERIVED PALO-ALTO LINES OF PLASMODIUM-FALCIPARUM ARE GENETICALLY UNRELATED
FANDEUR, T
BONNEFOY, S
MERCEREAUPUIJALON, O
[J]. MOLECULAR AND BIOCHEMICAL PARASITOLOGY, 1991, 47 (02) : 167 - 178
[10] TYPING OF SOUTHERN AFRICAN ISOLATES OF PLASMODIUM-FALCIPARUM USING MONOCLONAL-ANTIBODIES
FREESE, JA
MARKUS, MB
GOLENSER, J
[J]. AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE, 1990, 43 (01) : 19 - 24

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