BUFFER AND PH-DEPENDENCE OF THE RETENTION OF PHENYLTHIOCARBAMYLAMINO ACIDS IN REVERSED-PHASE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY

被引：17

作者：

MORVAI, M ^{[1
]}

FABIAN, V ^{[1
]}

MOLNARPERL, I ^{[1
]}

机构：

[1] L EOTVOS UNIV,INST INORGAN & ANALYT CHEM,POB 32,H-1518 BUDAPEST 112,HUNGARY

来源：

JOURNAL OF CHROMATOGRAPHY | 1992年 / 600卷 / 01期

关键词：

D O I：

10.1016/0021-9673(92)85441-U

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

An exhaustive study was made to find the optimum conditions for the determination of phenylthiocarbamyl (PTC)-amino acids. The effects of the type of salt dissolved in the eluent (sodium or ammonium acetate), the pH of the eluent, the presence of additives such as triethylamine and phosphate and the maximum storage time of the dissolved derivatives were investigated. On Hypersil or Nucleosil columns (15 cm x 4.6 mm I.D.; 5-mu-m) the determination of 21 PTC-amino acids [including cyst(e)ine, tryptophan and ornithine] require 22 min. The reproducibility of the measurments was < 5.0% (relative standard deviation).

引用

页码：87 / 92

页数：6

共 18 条

[1] USE OF PICO-TAG METHODOLOGY IN THE CHEMICAL-ANALYSIS OF PEPTIDES WITH CARBOXYL-TERMINAL AMIDES [J].

BENNETT, HPJ ;

SOLOMON, S .

JOURNAL OF CHROMATOGRAPHY, 1986, 359 :221-230

[2] RAPID ANALYSIS OF AMINO-ACIDS USING PRE-COLUMN DERIVATIZATION [J].

BIDLINGMEYER, BA ;

COHEN, SA ;

TARVIN, TL .

JOURNAL OF CHROMATOGRAPHY, 1984, 336 (01) :93-104

[3]

BIDLINGMEYER BA, 1986, METHODS PROTEIN SEQU

[4] AMINO-ACID ANALYSIS UTILIZING PHENYLISOTHIOCYANATE DERIVATIVES [J].

COHEN, SA ;

STRYDOM, DJ .

ANALYTICAL BIOCHEMISTRY, 1988, 174 (01) :1-16

[5] AMINO-ACID-ANALYSIS BY HPLC - OPTIMIZED CONDITIONS FOR CHROMATOGRAPHY OF PHENYLTHIOCARBAMYL DERIVATIVES [J].

EBERT, RF .

ANALYTICAL BIOCHEMISTRY, 1986, 154 (02) :431-435

[6]

ELKIN RG, 1987, CEREAL CHEM, V64, P226

[7] STABILITY OF PHENYL(THIOCARBAMOYL) AMINO-ACIDS AND OPTIMIZATION OF THEIR SEPARATION BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY [J].

GUITART, A ;

ORTE, PH ;

CACHO, J .

ANALYST, 1991, 116 (04) :399-403

[8] HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC DETERMINATION OF SELECTED AMINO-ACIDS IN RAT-BRAIN BY PRECOLUMN DERIVATIZATION WITH PHENYLISOTHIOCYANATE [J].

GUNAWAN, S ;

WALTON, NY ;

TREIMAN, DM .

JOURNAL OF CHROMATOGRAPHY, 1990, 503 (01) :177-187

[9]

HEINRIKSON RL, 1984, ANAL BIOCHEM, V136, P65, DOI 10.1016/0003-2697(84)90307-5

[10] HPLC ANALYSIS OF PHENYLTHIOCARBAMYL (PTC) AMINO-ACIDS .2. APPLICATION IN THE ANALYSIS OF (POLY)PEPTIDES [J].

JANSSEN, PSL ;

VANNISPEN, JW ;

MELGERS, PATA ;

VANDENBOGAART, HWM ;

VANAALST, GWM ;

GOVERDE, BC .

CHROMATOGRAPHIA, 1986, 22 (7-12) :351-357

← 1 2 →