A PARTICLE ACCELERATING DEVICE FOR DELIVERING DNA MATERIAL INTO PLANT-CELLS

被引：1

作者：

BUCKLEY, DJ ^{[1
]}

BROWN, DCW ^{[1
]}

LEFEBVRE, M ^{[1
]}

MCGRATH, A ^{[1
]}

TIAN, L ^{[1
]}

机构：

[1] AGR CANADA,PLANT RES CTR,RES BRANCH,OTTAWA,ON K1A 0C6,CANADA

来源：

COMPUTERS AND ELECTRONICS IN AGRICULTURE | 1995年 / 13卷 / 01期

关键词：

DNA TRANSFER; PARTICLE BOMBARDMENT; ELECTRONIC CONTROLLER;

D O I：

10.1016/0168-1699(95)00015-V

中图分类号：

S [农业科学];

学科分类号：

09 ;

摘要：

A particle acceleration device for implanting DNA material into plant cells using a timed burst of pressurized helium gas was developed. The device did not require the use of consumable materials (other than helium and microparticles), as used in some similar systems, and was thus easier and less expensive to operate. The system was designed to provide more precise control of the duration of the helium burst and of pressure differentials to ensure more reproducible results. Safety interlock circuitry was incorporated. The device was found to be efficient for delivery of DNA into alfalfa and soybean cell cultures.

引用

页码：67 / 74

页数：8

共 14 条

[1] DEVELOPMENT OF A SIMPLE PARTICLE BOMBARDMENT DEVICE FOR GENE-TRANSFER INTO PLANT-CELLS [J].

BROWN, DCW ;

TIAN, LN ;

BUCKLEY, DJ ;

LEFEBVRE, M ;

MCGRATH, A ;

WEBB, J .

PLANT CELL TISSUE AND ORGAN CULTURE, 1994, 37 (01) :47-53

[2]

CHRISTOU P, 1993, IN VITRO CELL DEV-PL, V29P, P119

[3]

FINER JJ, 1992, PLANT CELL REP, V11, P323, DOI 10.1007/BF00233358

[4] DEVELOPMENT AND OPERATION OF 5 PARTICLE GUNS FOR INTRODUCTION OF DNA INTO PLANT-CELLS [J].

GRAY, DJ ;

FINER, JJ .

PLANT CELL TISSUE AND ORGAN CULTURE, 1993, 33 (03) :219-219

[5]

JEFFERSON R, 1988, GENETIC ENG PRINCIPL, P247

[6] BETA-GLUCURONIDASE FROM ESCHERICHIA-COLI AS A GENE-FUSION MARKER [J].

JEFFERSON, RA ;

BURGESS, SM ;

HIRSH, D .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1986, 83 (22) :8447-8451

[7]

JEFFERSON RA, 1987, PLANT MOL BIOL REP, V3, P387

[8] THE BIOLISTIC(R) PDS-1000 HE DEVICE [J].

KIKKERT, JR .

PLANT CELL TISSUE AND ORGAN CULTURE, 1993, 33 (03) :221-226

[9] HIGH-VELOCITY MICROPROJECTILES FOR DELIVERING NUCLEIC-ACIDS INTO LIVING CELLS [J].

KLEIN, TM ;

WOLF, ED ;

WU, R ;

SANFORD, JC .

NATURE, 1987, 327 (6117) :70-73

[10]

Miki B. L., 1993, P67

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