LACK OF EFFECT OF TRANSIENT ISCHEMIA ON UBIQUITIN CONJUGATION

被引：6

作者：

KAMIKUBO, T ^{[1
]}

HAYASHI, T ^{[1
]}

OHKAWA, K ^{[1
]}

机构：

[1] JIKEI UNIV,SCH MED,DEPT BIOCHEM,MINATO KU,TOKYO 105,JAPAN

来源：

NEUROCHEMICAL RESEARCH | 1995年 / 20卷 / 04期

关键词：

UBIQUITIN; ISCHEMIA; CORTEX; HIPPOCAMPUS; GERBIL;

D O I：

10.1007/BF00973092

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Ubiquitin-conjugating activities in the soluble fractions of gerbil cortex and hippocampus following transient ischemia were examined in vitro. Ten minutes of ischemia did not affect the ubiquitination of heat-denatured lysozyme both in the cortex and in the hippocampus. No reduction of the conjugating activities following ischemia was also confirmed using the partially purified ubiquitin conjugating enzymes from the cortex. These results indicate that protein ubiquitination might not be impaired following transient ischemia.

引用

页码：391 / 394

页数：4

共 16 条

[1] BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3
[2] DEGRADATION OF NUCLEAR ONCOPROTEINS BY THE UBIQUITIN SYSTEM INVITRO
CIECHANOVER, A
DIGIUSEPPE, JA
BERCOVICH, B
ORIAN, A
RICHTER, JD
SCHWARTZ, AL
BRODEUR, GM
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1991, 88 (01) : 139 - 143
[3] ATP-DEPENDENT CONJUGATION OF RETICULOCYTE PROTEINS WITH THE POLYPEPTIDE REQUIRED FOR PROTEIN-DEGRADATION
CIECHANOVER, A
HELLER, H
ELIAS, S
HAAS, AL
HERSHKO, A
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1980, 77 (03): : 1365 - 1368
[4] GLOTZER M, 1991, NATURE, V349, P132, DOI 10.1038/349132a0
[5] HEMIN INHIBITS ATP-DEPENDENT UBIQUITIN-DEPENDENT PROTEOLYSIS - ROLE OF HEMIN IN REGULATING UBIQUITIN CONJUGATE DEGRADATION
HAAS, AL
ROSE, IA
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1981, 78 (11): : 6845 - 6848
[6] HAAS AL, 1988, J BIOL CHEM, V263, P13258
[7] CHANGES IN UBIQUITIN AND UBIQUITIN-PROTEIN CONJUGATES IN THE CA1 NEURONS AFTER TRANSIENT SUBLETHAL ISCHEMIA
HAYASHI, T
TAKADA, K
MATSUDA, M
[J]. MOLECULAR AND CHEMICAL NEUROPATHOLOGY, 1991, 15 (01) : 75 - 82
[8] INCREASE IN UBIQUITIN CONJUGATES DEPENDENT ON ISCHEMIC DAMAGE
HAYASHI, T
TANAKA, J
KAMIKUBO, T
TAKADA, K
MATSUDA, M
[J]. BRAIN RESEARCH, 1993, 620 (01) : 171 - 173
[9] POST-TRANSIENT ISCHEMIA INCREASE IN UBIQUITIN CONJUGATES IN THE EARLY REPERFUSION
HAYASHI, T
TAKADA, K
MATSUDA, M
[J]. NEUROREPORT, 1992, 3 (06) : 519 - 520
[10] SUBCELLULAR-DISTRIBUTION OF UBIQUITIN PROTEIN CONJUGATES IN THE HIPPOCAMPUS FOLLOWING TRANSIENT ISCHEMIA
HAYASHI, T
TAKADA, K
MATSUDA, M
[J]. JOURNAL OF NEUROSCIENCE RESEARCH, 1992, 31 (03) : 561 - 564

← 1 2 →