INHIBITORY EFFECT OF GENIPOSIDE ON AFLATOXIN-B(1)-INDUCED DNA-REPAIR SYNTHESIS IN PRIMARY CULTURED RAT HEPATOCYTES

被引：38

作者：

WANG, SW

LAI, CY

WANG, CJ

机构：

[1] CHUNG SHAN MED & DENT COLL,DEPT BIOCHEM,NO 113,SECT 2,TA CHING ST,TAICHUNG,TAIWAN

[2] CHUNG SHAN MED & DENT COLL,INST MED,TAICHUNG,TAIWAN

[3] CHUNG SHAN MED & DENT COLL,DEPT MED TECHNOL,TAICHUNG,TAIWAN

来源：

CANCER LETTERS | 1992年 / 65卷 / 02期

关键词：

GENIPOSIDE; AFLATOXIN-B(1); UNSCHEDULED DNA SYNTHESIS; GLUTATHIONE S-TRANSFERASE; GLUTATHIONE PEROXIDASE;

D O I：

10.1016/0304-3835(92)90157-Q

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

We have previously demonstrated that geniposide (GP) inhibits the aflatoxin B1 (AFB1) induced-hepatotoxicity and hepatic DNA binding in rats. To address the mechanism of action, the effects of GP on AFB1-induced DNA repair synthesis and AFB1 biotransformation in cultured rat hepatocytes were investigated. By evaluation of unscheduled DNA synthesis (UDS), GP reduced AFB1-induced DNA repair synthesis in a dose-dependent manner in hepatocyte cultures. GP elevates the metabolism of AFB1 and decreases the formation of AFM1. The enzyme activities of glutathione S-transferase (GST) and GSH-peroxidase (GSH-Px) in AFB1-treated hepatocyte cultures are enhanced in the presence of GP. GP reduces AFB1-induced DNA repair synthesis through an increased AFB1 detoxication metabolism. It provides one possible mechanism for the chemopreventive activity of GP.

引用

页码：133 / 137

页数：5

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