EXPRESSION OF THE OLFACTORY CYCLIC-NUCLEOTIDE GATED CHANNEL (CNG1) IN THE RAT-BRAIN

被引：24

作者：

ELHUSSEINI, AED ^{[1
]}

BLADEN, C ^{[1
]}

VINCENT, SR ^{[1
]}

机构：

[1] UNIV BRITISH COLUMBIA,DEPT PSYCHIAT,DIV NEUROL SCI,VANCOUVER,BC V6T 1Z3,CANADA

来源：

NEUROREPORT | 1995年 / 6卷 / 10期

关键词：

3'; 5'-CYCLIC GUANOSINE MONOPHOSPHATE; CYCLIC NUCLEOTIDE GATED CHANNEL SUBUNIT 1; REVERSE TRANSCRIPTION; DEOXYNUCLEOTIDE TRIPHOSPHATE;

D O I：

10.1097/00001756-199507100-00024

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

THE expression of the olfactory cyclic nucleotide-gated channel (CNG1) was studied in the rat brain. Using RT-PCR, levels of CNG1 mRNA were determined relative to the expression of a constantly expressed gene, ol-tubulin. RT-PCR showed that CNG1 mRNA was detectable in the pituitary gland, the olfactory bulb, and the cerebellum of adult and 5-day-old rats. A 3.4 kb mRNA was detected in the olfactory bulb by Northern blotting. In situ hybridization analysis showed that CNG1 mRNA expression is present in the olfactory bulb and in the Purkinje cells of the cerebellum. RT-PCR studies on Purkinje cell-enriched cultures obtained from the cerebellum of 16-day-old embryos (E16) confirmed the expression of CNG1 mRNA in these neurones. Our results show that CNG1 is not restricted to the olfactory epithelium but is also present in specific regions of the brain. These results suggest that cyclic nucleotides may act in the regions that possess CNG1 gene expression to affect the electrical activity of certain neurones directly.

引用

页码：1459 / 1463

页数：5

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