PHYSICOCHEMICAL AND IMMUNOLOGICAL CHARACTERIZATION OF RECOMBINANT HOST-PROTECTIVE ANTIGEN (VP2) OF INFECTIOUS BURSAL DISEASE VIRUS

被引：41

作者：

AZAD, AA ^{[1
]}

MCKERN, NM ^{[1
]}

MACREADIE, IG ^{[1
]}

FAILLA, P ^{[1
]}

HEINE, HG ^{[1
]}

CHAPMAN, A ^{[1
]}

WARD, CW ^{[1
]}

FAHEY, KJ ^{[1
]}

机构：

[1] CSIRO,DIV ANIM HLTH,PARKVILLE,VIC 3052,AUSTRALIA

来源：

VACCINE | 1991年 / 9卷 / 10期

关键词：

INFECTIOUS BURSAL DISEASE VIRUS; RECOMBINANT ANTIGEN; ESCHERICHIA-COLI; YEAST; N-TERMINAL FUSIONS; IMMUNE RESPONSE;

D O I：

10.1016/0264-410X(91)90286-F

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Small fusions to the N-terminal end of the host-protective antigen (VP2) of infectious bursal disease virus lead to stable expression of VP2 in Escherichia coli and yeast, and reduce the levels of inclusion body formation in E. coli in comparison to VP2 constructs with larger N-terminal fusions. VP2 produced with small N-terminal fusions, like native viral VP2, can be fractionated into a high molecular weight 'multimeric' form and a monomeric form. A virus-neutralizing monoclonal antibody that only recognizes undenatured VP2 preferentially reacts with multimeric forms of recombinant VP2. Both native and recombinant monomeric forms of VP2 are non-immunogenic. The multimeric forms of viral and yeast-derived VP2 are highly immunogenic, while those produced in E. coli are not.

引用

页码：715 / 722

页数：8

共 34 条

[1] DELETION MAPPING AND EXPRESSION IN ESCHERICHIA-COLI OF THE LARGE GENOMIC SEGMENT OF A BIRNAVIRUS [J].