MOLECULAR KARYOTYPE OF RELATED CLONES OF ENTAMOEBA-HISTOLYTICA

被引：17

作者：

OROZCO, E ^{[1
]}

BAEZCAMARGO, M ^{[1
]}

GAMBOA, L ^{[1
]}

FLORES, E ^{[1
]}

VALDES, J ^{[1
]}

HERNANDEZ, F ^{[1
]}

机构：

[1] IPN, CTR INVEST & ESTUDIOS AVANZADOS, DEPT PHYSIOL & BIOPHYS, MEXICO CITY 07000, DF, MEXICO

来源：

MOLECULAR AND BIOCHEMICAL PARASITOLOGY | 1993年 / 59卷 / 01期

关键词：

ENTAMOEBA-HISTOLYTICA; CHROMOSOMAL POLYMORPHISM; GENETICALLY RELATED CLONES; CLONAL REPRODUCTION;

D O I：

10.1016/0166-6851(93)90004-H

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The molecular karyotype of 3 clones derived from strain HM1:IMSS of Entamoeba histolytica was studied by transverse alternating field electrophoresis. 11-20 bands ranging between 0.3 and over 3 Mb were resolved. Hybridization with total DNA detected highly repetitive sequences in the slow-migrating molecules, while non-repetitive sequences were located in the intermediate and fast-migrating molecules. rDNA, tubulin, actin, cysteine proteases DNA fragments, and a variable DNA sequence (EhVR1) located the respective genes mainly in the 1.3-1.5-Mb region, although they differed in the three clones. Two-dimensional transverse alternating field electrophoresis showed that more than one high-molecular weight molecule may comigrate in a single DNA band. rDNA, and EhVR1 hybridized with slow-migrating bands in a characteristic ladder pattern. Most of the bands recognized by EhVR1 seems to be linear molecules, although exonuclease III-resistant bands also hybridized with EhVR1, suggesting the presence of circles.

引用

页码：29 / 40

页数：12

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