GLYCOGENOLYTIC EFFECT OF PANCREASTATIN IN ISOLATED RAT HEPATOCYTES IS MEDIATED BY A CYCLIC-AMP-INDEPENDENT CA2+-DEPENDENT MECHANISM

被引：43

作者：

SANCHEZ, V ^{[1
]}

LUCAS, M ^{[1
]}

CALVO, JR ^{[1
]}

GOBERNA, R ^{[1
]}

机构：

[1] UNIV SEVILLA,SCH MED,DEPT MED BIOCHEM & MOLEC BIOL,AV SANCHEZ PIZJUAN 4,E-41009 SEVILLE,SPAIN

来源：

BIOCHEMICAL JOURNAL | 1992年 / 284卷

关键词：

D O I：

10.1042/bj2840659

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have studied the effect of pig pancreastatin on glucose and lactate production in freshly isolated rat hepatocytes. Pancreastatin stimulated the rate of glucose output, whereas, in contrast with glucagon, it failed to modify the rate of lactate production. The effective concentration of pancreastatin was in the range 0.1-100 nM, with half-maximal rate close to 1 nM. The ability of pancreastatin to increase glucose output was abolished by chelation of the calcium in the medium. By itself, pancreastatin did not increase cyclic AMP (cAMP) levels and had no influence on cAMP levels in glucagon-stimulated hepatocytes. Our results point out a possible role of pancreastatin in glycogenolysis. This appears to be mediated by a cAMP-independent Ca2+-dependent mechanism.

引用

页码：659 / 662

页数：4

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