A NEW CHEMILUMINOMETRIC METHOD FOR THE DETERMINATION OF GLYCEROL IN WINE BY FLOW-INJECTION ANALYSTS WITH IMMOBILIZED GLYCEROL DEHYDROGENASE IN COMBINATION WITH NADH OXIDASE

被引：10

作者：

KONDRUWEIT, S ^{[1
]}

DREMEL, BAA ^{[1
]}

SCHMID, RD ^{[1
]}

机构：

[1] GESELL BIOTECHNOL FORSCH MBH,D-38124 BRAUNSCHWEIG,GERMANY

来源：

ANALYTICAL LETTERS | 1994年 / 27卷 / 08期

关键词：

FIA; GLYCEROL; GLYCEROL DEHYDROGENASE; NADH OXIDASE; CHEMILUMINESCENCE; FLUORESCENCE;

D O I：

10.1080/00032719408006384

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A new chemiluminometric method for the determination of glycerol in wine by FIA is presented. The method is based on the combination of immobilised glycerol dehydrogenase and NADH oxidase. It is compared with common methods of glycerol determinations in wine. In contrast to chemical determinations the chemiluminometric method is highly specific and in contrast to fluorometric determinations it is not disturbed by sample self-fluorescence. The automated FIA set-up allows glycerol determinations in wine from 0.1 - 10 mM without sample pretreatment; the results are in good correlation to those obtained with official reference methods. The generic detection system allows an easy adaptation to other analytical tasks based on dehydrogenase reactions.

引用

页码：1489 / 1505

页数：17

共 22 条

[1] BIAMPEROMETRIC DETERMINATION OF GLYCEROL AND TRIGLYCERIDES WITH OPEN TUBULAR CARBON ELECTRODES IN FLOW STREAMS [J].

ATTIYAT, AS ;

CHRISTIAN, GD .

ANALYTICA CHIMICA ACTA, 1979, 106 (02) :225-231

[2]

BERGMEYER HU, 1988, METHOD ENZYMAT AN, V4, P504

[3]

BIER F, 1991, Patent No. 1214

[4]

BLUM LJ, 1988, ANAL LETT, V21, P717

[5] OPTICAL SENSORS FOR BIOPROCESS CONTROL [J].

DREMEL, BAA ;

SCHMID, RD .

CHEMIE INGENIEUR TECHNIK, 1992, 64 (06) :510-517

[6]

DREMEL BAA, 1992, THESIS AACHEN, P16

[7]

FONONG T, 1987, ANAL LETT, V20, P783

[8] AUTOMATED ENZYMIC METHOD FOR DETERMINATION OF GLYCEROL [J].

GORE, MG .

ANALYTICAL BIOCHEMISTRY, 1976, 75 (02) :604-610

[9]

HECHT HJ, 1993, UNPUB J MOL BIOL

[10] COMPUTER ACQUISITION AND ANALYSIS OF DATA IN ENZYMATIC-FLUOROMETRIC-CONTINUOUS-FLOW METHODS FOR THE MEASUREMENT OF GLUCOSE, LACTATE, PYRUVATE, ALANINE, GLYCEROL AND 3-HYDROXYBUTYRATE IN HUMAN-BLOOD [J].

HETHERINGTON, CS ;

KEIR, M ;

STAPPENBECK, R ;

SIMM, A ;

BRIGHAM, LL ;

SKILLEN, AW ;

HODSON, AW .

JOURNAL OF AUTOMATIC CHEMISTRY, 1986, 8 (02) :49-55

← 1 2 3 →