THE ALPHA-ISOENZYME AND PI-ISOENZYME OF GLUTATHIONE S-TRANSFERASE IN HUMAN FETAL LUNG - INUTERO ONTOGENY COMPARED WITH DIFFERENTIATION IN LUNG ORGAN-CULTURE

Polyclonal antisera to the α and π isoenzymes of glutathione S-transferase have been used in immunohistochemical studies of developing human lung. In utero expression of the π set was down-regulated in distal airway cells and the first appearance of π-negative cells coincided with phenotypic differentiation. In contrast, in the early phase of fetal lung organ culture π isoenzyme was detected in all differentiated epithelial cells and only as culture progressed did focal negativity develop. The α set showed no developmental changes in utero or in organ culture. © 1990.