DIFFERENTIAL GENE-EXPRESSION DURING SPORULATION IN BACILLUS-SUBTILIS - STRUCTURE AND REGULATION OF THE SPOIIID GENE

被引:40
作者
STEVENS, CM [1 ]
ERRINGTON, J [1 ]
机构
[1] UNIV OXFORD,DEPT BIOCHEM,MICROBIOL UNIT,OXFORD OX1 3QU,ENGLAND
关键词
D O I
10.1111/j.1365-2958.1990.tb00622.x
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The gene spoIIID, which is essential for spore formation in Bacillus subtilis, was cloned and sequenced. It consists of one open reading frame which would encode a 93‐amino‐acid protein with a classic helix‐turn‐helix motif, characteristic of sequence‐specific DNA‐binding proteins. SpoIIID protein is a previously identified transcription factor, capable of altering the specificity of RNA polymerase containing sigma K in vitro (Kroos et al., 1989). The spoIIID83 mutation (by which the locus was originally identified), was sequenced and found to be a single base substitution in the ribosome binding site upstream of the spoIIID open reading frame. A transcriptional fusion to lacZ was constructed and used to examine the regulation of spoIIID. Expression of spoIIID occurred only during sporulation, beginning 1.5 to 2 hours after the initiation of sporulation. The dependence of spoIIID expression on other spo loci suggests that it is mother‐cell‐specific, and that it is transcribed by sigma E‐containing RNA polymerase. Copyright © 1990, Wiley Blackwell. All rights reserved
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页码:543 / 551
页数:9
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