SYNTHESIS OF 2-0-ALPHA-D-GLUCOPYRANOSYL L-ASCORBIC-ACID BY CYCLOMALTODEXTRIN GLUCANOTRANSFERASE FROM BACILLUS-STEAROTHERMOPHILUS

被引：103

作者：

AGA, H ^{[1
]}

YONEYAMA, M ^{[1
]}

SAKAI, S ^{[1
]}

YAMAMOTO, I ^{[1
]}

机构：

[1] OKAYAMA UNIV,FAC PHARMACEUT SCI,DEPT IMMUNOCHEM,OKAYAMA 700,JAPAN

来源：

AGRICULTURAL AND BIOLOGICAL CHEMISTRY | 1991年 / 55卷 / 07期

关键词：

D O I：

10.1080/00021369.1991.10870856

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

Cyclomaltodextrin glucanotransferase (CGTase) [EC 2.4.1.19] was found to catalyze the transglycosylation from alpha-cyclodextrin (alpha-CD) to L-ascorbic acid (AA). A main product formed by this reaction was identified as 2-O-alpha-D-glucopyranosyl L-ascorbic acid (AA-2G) with the enzymatic hydrolysis and ultraviolet absorption spectra using standard AA-2G. A series of maltooligosaccharide substituted 2-O-derivatives of AA were also detected by HPLC. These were thoroughly hydrolized to AA-2G and glucose by treatment with glucoamylase [EC 3.2.1.3]. A large amount of AA-2G was prepared with 500 g of AA and 1000 g of alpha-CD. Two hundred and fifty grams of purified AA-2G, the content of which was 97%, was obtained through enzyme reactions and purification with HPLC using an ion-exchange resin.

引用

页码：1751 / 1756

页数：6

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